Web

• http://microbiology.korea.ac.kr
• http://club.cyworld.com/kube

Address

• Lab of Industrial Microbiology & Bioenergy, Room L206
• Lab of Metabolic Engineering & Biorefinery, Room 205
• Life Science Building (West Building), Room 208 (Office)
• Division of Biotechnology (undergraduate)
• School of Life Sciences & Biotechnology (graduate)
• College of Life Sciences & Biotechnology
• Korea University

Education

Experience

Professional Activities

• -    Korean Institute of Chemical Engineers (Biochemical Engineering Division), Secretary of Organization
• -    The Microbiological Society of Korea, Secretary of Publication Committee
• -    Korean Society for Biotechnology and Bioengineering, Secretary
• -    Editorial Board of Biotechnology and Bioprocess Engineering
• -    Korean Society for Microbiology and Biotechnology, Member
• -    The American Society for Microbiology, Member
• -    The Australian Society for Microbiology, Member

• -       Kang DH, Hyeon JE, You SK, Kim SW, Han SO*. (2014) Efficient enzymatic degradation process for hydrolysis activity of the carrageenan from red algae in marine biomass. Journal of Biotechnology.192:108-113




• -       Kim SJ, Hyeon JE, Jeon SD, Choi G, Han SO*. (2014) Bi-functional c ellulases complexes displayed on the cell surface of Corynebacterium glutamicum increase hydrolysis of lignocelluloses at elevated temperature. Enzyme and Microbial Technology. 66:67-73




• -       Hyeon JE, Kang DH, Han SO*. (2014) Signal a mplification by a self-assembled biosensor system designed on the principle of dockerin-cohesin interactions in a cellulosome complex . Analyst . 139:4790-4793


• -             Hyeon JE, You SK, Kang DH, Ryu S, Kim M, Lee S , Han SO*. (2014) Enzymatic d egradation of l ignocellulosic b iomass by c ontinuous p rocess u sing l accase and c ellulases with the a id of s caffoldin for e thanol p roduction . Process Biochemistry . 49:1266-1273


• -             Hyeon JE, Jeon SD , Han SO*. (2013) Cellulosome-based, Clostridium -derived multi-functional enzyme complexes for advanced biotechnology tool development . Biotechnology Advances. 31:936–944


• -             Jeon SD, Lee JE, Kim SJ, Park SH, Choi G, Han SO*. (2013) Unique contribution of the cell wall-binding endoglucanase G to the cellulolytic complex in Clostridium cellulovorans . Applied and Environmental Microbiology. 79:5942-5948


• -             Yu KO, Jung J, Ramzi AB, Choe SH, Kim SW, Park C, Han SO*. (2013) Development of a Saccharomyces cerevisiae s train for i ncreasing the a ccumulation of t riacylglycerol as a m icrobial o il f eedstock for b iodiesel p roduction using g lycerol as a s ubstrate . Biotechnology and Bioengineering. 110:343-347


• -             Go A, Lee Y, Kim Y, Park S, Choi J, Lee J, Han SO , Kim SW, Park C. (2013) Enzymatic coproduction of biodiesel and glycerol carbonate from soybean oil in solvent-free system . Enzyme and Microbial Technology. 53:154-158


• -             Jeon SD, Lee JE, Kim SJ, Kim SW, Han SO*. (2012) Analysis of selective, high protein-protein binding interaction of cohesin-dockerin complex using biosensing methods. Biosensors and Bioelectronics. 35:382-389


• -             Hyeon JE, Kang DH, Kim YI, You SK, Han SO*. (2012) A GntR type transcriptional regulator PckR negatively regulates the expression of phosphoenolpyruvate carboxykinase in Corynebacterium glutamicum . Journal of Bacteriology. 194:2181-2188


• -             Yu KO, Jung J, Kim SW, Park CH, Han SO*. (2012) Synthesis of FAEEs from glycerol in engineered Saccharomyces cerevisiae using endogenously produced ethanol by heterologous-expression of an unspecific bacterial acyltransferase. Biotechnology and Bioengineering. 109:110-115


• -             Jung J, Yu KO, Ramzi AB, Choe SH, Kim SW, Han SO*. (2012) Improvement of surfactin production in Bacillus subtilis using synthetic wastewater by overexpression of specific extracellular signaling peptides, comX and phrC . Biotechnology and Bioengineering. 109:2349-2356


• -             Hyeon JE, Kang DH, Kim YI, Jeon SD, You SK, Kim KY, Kim SW, Han SO*. (2012) Production of functional agarolytic nano-complex for the synergistic hydrolysis of marine biomass and its potential application in carbohydrate binding module-utilizing one-step purification. Process Biochemistry. 47:877-881



• -             Yu KO, Jung J, Ramzi AB, Choe SH, Kim SW, Park C, Han SO*. (2012) Increased enthanol production from glycerol by Saccharomyces cerevisiae strains with enhanced stress tolerance from the overexpression of SAGA complex components. Enzyme and Microbial Technology. 35:382-389


• -             Jeon SD, Yu KO, Kim SW, Han SO*. (2012) The processive endoglucanase EngZ is active in crystalline cellulose degradation as a cellulosomal subunit of Clostridium cellulovorans . New Biotechnology. 29:365-371


• -             Yu KO, Jung J, Ramzi AB, Kim SW, Park C, Han SO*. (2012) Improvement of ethanol yield from glycerol via conversion of pyruvate to ethanol in metabolically engineered Saccharomyces cerevisiae . Applied Biochemistry and Biotechnology. 166:856-865


• -             Lee SJ, Kim SB, Kang SW, Han SO , Park C, Kim SW. (2012) Effect of crude glycerol-derived inhibitors on ethanol production by Enterobacter aerogenes . Biotechnology and Bioprocess Engineering. 35:85-92


• -             Kim SB, Lee SJ, Jang EJ, Han SO , Park C, Kim SW. (2012) Sugar recovery from rice straw by dilute acid pretreatment. Journal of Industrial and Engineering Chemistry. 18:183-187


• -             Kim E, Song YS, Choi HS, Yoo HY, Kang SW, Song KH, Han SO , Kim SW. (2012) Efficient immobilization technique for enhancement of cellobiose dehydrogenase activity on silica gel. Biotechnology and Bioprocess Engineering. 17:55-59


• -             Jeon E, Lee S, Lee S, Han SO , Yoon YJ, Lee J. (2012) Improved production of long-chain fatty acid in Escherichia coli by an engineering elongation cycle during fatty acid synthesis (FAS) through genetic manipulation. Journal of Microbiology and Biotechnology. 22:990-999.


• -             Jung H, Lee Y, Kim D, Han SO , Kim SW, Lee J, Kim YH, Park C. (2012) Enzymatic production of glycerol carbonate from by-product after biodiesel manufacturing process. Enzyme and Microbial Technology . 51:143-147


• -             Go A , Ko JW, Lee SJ, Kim SW, Han SO , Lee J, Han MW, Um Y, Nam J, Park C. (2012) Process Design and Evaluation of Value-added Chemicals Production from Biomass. Biotechnology and Bioprocess Engineering. 17:1055-1061


• -             Seo TY, Eum KW, Han SO , Kim SW, Kim JH, Song KH, Choe J. (2012) Immobilized cell microchannel bioreactor for evaluating fermentation characteristics of mixed substrate consumption and product formation . Process Biochemistry. 47:1011-1015


• -             Hyeon JE, Jeon WJ, Whang SY, Han SO*. (2011) Production of minicellulosomes for the enhanced hydrolysis of cellulosic substrates by recombinant Corynebacterium glutamicum . Enzyme and Microbial Technology . 48:371-377


• -             Jeon SD, Yu KO, Kim SW, Han SO*. (2011) A celluloytic complex from Clostridium cellulovorans consisting of cellulosomal mannanase B and endoglucanase E has synergistic effects on galactomannan degradation. Applied Microbiology and Biotechnology. 90:565-572


• -             Jeon E, Lee S, Won JI, Han SO , Kim J, Lee J. (2011) Development of Escherichia coli MG1655 strains to produce long chain fatty acids by engineering fatty acid synthesis (FAS) metabolism. Enzyme and Microbial Technology. 49:44-51


• -             Lee JH, Kim SB, Kang SW, Song YS, Park C, Han SO , Kim SW. (2011) Biodiesel production by a mixture of Candida rugosa and Rhizopus oryzae lipases using a supercritical carbon dioxide process. Bioresource Technology . 102:2105-2108


• -             Seong PJ, Jeon BW, Lee M, Cho DH, Kim DK, Jung KS, Kim SW, Han SO , Kim YH, Park C. (2011) Enzymatic coproduction of biodiesel and glycerol carbonate from soybean oil and dimethyl carbonate. Enzyme and Microbial Technology . 48:505-509


• -             Lee H, Cho H, Kim YH, Shin SJ, Kim SB, Han SO , Lee J, Kim SW, Park C. (2011) Tolerance of Saccharomyces cerevisiae K35 to lignocellulose-derived inhibitory compounds. Biotechnology and Bioprocess Engineering. 16:755-760


• -             Cho HY, Lee SG, Hyeon JE, Han SO*. (2010) Identification and characterization of a transcriptional regulator, SucR, that influences sucCD transcription in Corynebacterium glutamicum . Biochemical and Biophysical Research Communications . 401:300-305


• -             Yu KO, Kim SW, Han SO*. (2010) Reduction of glycerol production to improve ethanol yield in an engineered Saccharomyces cerevisiae using glycerol as a substrate. Journal of Biotechnology . 150:209-214


• -             Hyeon JE, Yu KO, Suh DJ, Suh YW, Lee SE, Lee J, Han SO*. (2010) Production of minicellulosomes from Clostridium cellulovorans for the fermentation of cellulosic ethanol using engineered recombinant Saccharomyces cerevisiae . FEMS Microbiology Letter. 310: 39-47


• -             Yu KO, Kim SW, Han SO*. (2010) Engineering of glycerol utilization pathway for ethanol production by Saccharomyces cerevisiae . Bioresource Technology . 101:4157-4161


• -             Lee CY, Yu KO, Kim SW, Han SO*. (2010) Enhancement of the thermostability and activity on mesophilic Clostridium cellulovorans EngD by in vitro DNA recombination with Clostridium thermocellum CelE. Journal of Bioscience and Bioengineering . 109:331-336


• -             Cho W, Jeon SD, Shim HJ, Doi RH, Han SO*. (2010) Cellulosomic profiling produced by Clostridium cellulovorans during growth on different carbon sources explored by the cohesin marker. Journal of Biotechnology . 145:233-239


• -             Lee SJ, Oh EK, Oh YH, Won JI, Han SO , Lee JW. (2010) Increased ethanol resistance in ethanolic Escherichia coli by insertion of heat-shock genes BEM1 and SOD2 from Saccharomyces cerevisiae .Biotechnology and Bioprocess Engineering. 15:770-776


• -             Lee JH, Kim SB, Park C, Tae B, Han SO , Kim SW. (2010) Development of batch and continuous processes on biodiesel production in a packed-bed reactor by a mixture of immobilized Candida rugosa and Rhizopus oryzae lipases. Applied Biochemistry and Biotechnology . 161:365-371


• -             Lee SM, Jin LH, Kim JH, Han SO , Na HB, Hyeon T, Koo YM, Kim J, Lee JH. (2010) β-Glucosidase coating on polymer nanofibers for improved cellulosic ethanol production. Bioprocess and Biosystems Engineering . 33:141-147


• -             Jeon E, Hyeon JE, Suh DJ, Suh YW, Kim SW, Song KH, Han SO*. (2009) Production of cellulosic ethanol in Saccharomyces cerevisiae heterologous expressing Clostridium thermocellum endoglucanase and Saccharomycopsis fibuligera β-glucosidase genes. Molecules and Cells . 28:369-373


• -             Jeon E, Hyeon JE, Lee SE, Park BS, Kim SW, Lee J, Han SO*. (2009) Cellulosic alcoholic fermentation using recombinant Saccharomyces cerevisiae engineered for the production of Clostridium cellulovorans endoglucanase and Saccharomycopsis fibuligera beta β-glucosidase. FEMS Microbiology Letter . 301:130-136

   *Corresponding Author

Research Topic

Based on “ Molecular Microbiology and Biotechnology ”, our laboratory is seeking solutions in tackling energy and environmental issues by taking advantages of the useful bioconversion capability of microorganisms and their enzymes. We are currently working on studying fundamental novel scientific knowledge and developing technologies for the conversion of renewable biomass to value-added chemicals, including biofuels, gasification, enzymes and fermentation.

We work as a team among three teams, and have a very good organization. Here's a snapshot of each team’s research activities.

Team I: Designer Microbe

• - Strain design using metabolic reconstruction to produce desired products
• - Improvement of enzyme, transport and regulatory functions of the cell by the application of recombinant DNA technologies
• - Optimizing genetic and regulatory processes within cells
• - Directly deleting and overexpressing the genes that encode for metabolic enzymes
• - 대사공학 및 시스템생물공학 기반 바이오화합물 , 바이오연료 대량 생산 균주 연구
• - 합성 생물학기반 신규 유용 생리 활성물질 대량 생산 기술 연구

Team II: Designer Enzymes

• - Nano-Arm Engineering - Cell Surface Display
• - Industrial Recombinant - Fermentation Process
• - Regulator Control: Novel Switching System
• - 세포 표면 디스플레이 시스템을 응용한 나노암 (Nano-Arm) 탑재 슈퍼 산업 균주 개발
• - 차세대 유전체 기능 분석 및 대사제어를 통한 미생물 오믹스 (Omics) 정보 시스템 연구

Team III: Cellulosomics

• - Formation of Nano-Complex, Cellulosome and Biological expression
• - Cellulase Detection: Fungal Cellulase and Bacteria Cellulase
• - Improvement of Cellulase Efficiency: Production and Recombinant Cellulase
• - 나노 컴플렉스 셀룰로좀 형성 기작 규명 및 생물학적 응용
• - 신규 셀룰레이즈 및 분해효소 동정 : 박테리아 및 곰팡이 유래 효소
• - 분해효소 효율성 극대화 : 효소 생산 및 돌연변이를 이용한 재조합

바이오 연료 및 바이 오 화학 산업은 미래 지향적이고 환경친화적인 바이오 기술 이다 . 바이오 연료 및 화학 제품의 산업화를 위해서는 저비용의 환경친화적인 바이오매스를 사용하여 경제성 있는 공정에 대한 과학적 접근이 필요하다 . 따라서 본 연구실에서는 효율적인 당화를 위한 시스템을 구축하고 , 미생물의 대사 경로를 조절하여 특정 기능을 제어하거나 증폭시킬 수 있는 산업 균주를 개발하여 대량 생산이 가능하게 하는 연구가 진행중에 있다 .

경제적인 바이오 연료 생산을 목적으로 목질계는 물론 해양계 바이오 매스로부터 슈가플랫폼을 구축하기 위하여 당화 균주를 개발 중이며 , 효과적인 분해를 위한 효소공학 및 나노융합 기술을 응용하여 고효율의 효소를 개발하고 있다 . 목질계나 해양계 바이오 매스를 바이오 에탄올 생산에 이용하기 위해 분해 효율이 높은 Enzyme nano-complex( 효소나노복합체 ) 인 cellulosome 의 형성 기작과 cohesin-dockerin 상호 작용을 응용하는 연구가 진행하고 있으며 , 이 원리를 이용한 minicellulosome 의 생성 및 응용 연구가 이루어지고 있다 . 또한 세포 표면 디스플레이 시스템을 응용한 나노암 (Nano-arm) 탑재 슈퍼 산업 균주의 개발을 통하여 공정을 최적화 시키는 시도와 차세대 유전체 기능 분석 및 대사제어를 통한 미생물 오믹스 (Omics) 정보 시스템을 개발하고 있다 . 미생물을 이용한 바이오에너지 및 바이오화합물를 생산하기 위하여 대사공학 및 시스템생물공학을 기반으로 하는 균주 개발의 중요성이 대두됨에 따라 인위적으로 세포의 대사과정을 조절하여 바이오화합물 및 바이오 연료와 같은 유용 생물소재의 대량생산을 위한 연구를 진행하고 있다 .

본 연구실은 국내 바이오리파이너리의 선도적인 연구실로서 자리잡기 위하여 총 세팀으로 구성이 되어 연구를 진행 중에 있으며 연구실을 구성하는 세 팀들은 미생물의 물질대사적 원리를 밝히고 이를 대사공학을 기반으로 응용한 유용한 균주의 개발 , 효소에 대한 유전학 및 생화학 기반의 과학적 원리를 기반으로 바이오매스의 활용을 위한 고효율의 효소 개발 , 효소 복합체인 셀룰로좀 (Cellulosome) 의 원리를 밝히는 오믹스 (Omics) 개념의 Cellulosomics 학문 연구를 각각 목표로 하고 있다 . 세 팀간의 유기적인 연구 및 연구 결과의 내부적인 공유를 통하여 저비용의 환경친화적인 바이오매스를 사용하여 경제성 있는 공정에 대하여 과학적으로 접근하고 있다 . 따라서 , 본 연구진에서는 바이오매스의 효율적인 당화를 통하여 저비용의 당을 확보하고 바이오리파이너리 최종 생산물인 바이오 연료 및 고부가가치 바이오화합물 생산을 위한 미생물 균주개량에 대한 시스템 개발 , 신규 미생물 탐색 , 대사공학 및 단백질 공학을 이용한 발효 및 생산공정의 최적화 , Omics 등의 신규 툴을 이용하여 C2, C3, C4 등의 범용 화학소재 생산을 위한 플랫폼 미생물을 제작하고 이를 통하여 platform chemical 을 생산 하고 있다 .

궁극적으로 , 본 연구실에서는 바이오매스 유래의 각종 신규 기능성 단량체 , 고분자 중간체 , 비이오플라스틱 , 바이오에너지 및 고부가가치 정밀화학제품을 생산할 수 있는 핵심 기반기술을 개발하여 기존의 화학산업을 “ 저탄소 녹색성장 ” 산업으로 전환하는 국가적 비전에 부응하고자 한다 .

준비 중입니다.

Ph.D student

• -             Kyoung Ok You ( 유경옥 ), Ph.D course


• -             Soo Jung Kim ( 김수정 ), Ph.D course


• -             Sang Duk Jeon ( 전상덕 ), Integrated MS & Ph.D course


• -             Ahmad Bazli Ramzi ( 바즐리 ), Integrated MS & Ph.D course


• -             Jeong Eun Hyeon ( 현정은 ), Integrated MS & Ph.D course


• -             Dae Hee Kang ( 강대희 ), Integrated MS & Ph.D course


• -             Kyoung A Youn ( 윤경아 ), Integrated MS & Ph.D course


• -             Seung Kyou You ( 유승규 ), Integrated MS & Ph.D course

Master student

• -             Ju Jung ( 정주 ), MS course


• -             Se Hoon Choe ( 최세훈 ), MS course


• -             Young In Kim ( 김영인 ), MS course


• -             Ji Eun Lee ( 이지은 ), MS course


• -             Ji Eun Joo ( 주지은 ), MS course


• -             Thien The Tran ( 트랜 ), MS course


• -             Ji Hyoung Nam ( 남지형 ), MS course


• -             Seong Hyun Park ( 박성현 ), MS course

Research fellow

• -             Chang Youn Cho ( 조창연 ), Research assistant (Jr student)