Lab. of Molecular Biochemistry 분자생화학연구실

 생체스트레스 혹은 감염원 유발 질병들의 발생기전을 전사 및 단백질발현 조절 수준에서 규명하여, 질병치유 및 감염제어를 위한 기초 지식을 제공하고 궁극적으로 유전자치료에 기여함.

Education

• ∙ Ph.D.: Univ. of California, Davis, Biological Chemistry, USA, 1990
• ∙ Master: KAIST, Biological Science & Engineering, Korea, 1981
• ∙ Bachelor: Korea University, Food Science, Korea, 1978

Professional Experiences

• ∙ Professor, Division/Dept. of Life Sci., Korea University, Seoul, Korea, 1995-present
• ∙ Instructor, Boston University, MA, USA. 1992-1995
• ∙ Post-doc, Harvard Medical School, MA, USA. 1990-1992
• ∙ Researcher, Agency for Defense Development, Seoul, Korea, 1981-1984

• ‣ Shin, N., C.W. Pyo, K.I. Jung, and S.-Y. Choi, Influenza A virus PB1-F2 is involved in regulation of cellular redox state in alveolar epithelial cells, Biochem. Bioph. Res. Co., 459(4) (2015)
• ‣ Park, S.W., C.W. Pyo and S.-Y. Choi, High-efficiency lentiviral transduction of primary human CD34+ hematopoietic cells with low-dose viral inocula. Biotechnol. Lett., 37(2), 281-288 (2015)
• ‣ Hwang, Y.W., N.K. Yoo, H.M. Yang and S.-Y. Choi, RNA polymerase III control elements are required for trans-activation by the murine retroviral long terminal repeat sequences. Biochem. Bioph. Res. Co., 456(1), 110-115 (2015)
• ‣ Pyo, C.W., N. Shin, K.I. Jung, J.H. Choi, S.-Y. Choi, Alteration of copper-zinc superoxide dismutase 1 expression by influenza A virus is correlated with virus replication. Biochem. Bioph. Res. Co., 450(1), 711-716 (2014)
• ‣ Park, J.H., Y.J Jeong, H.K. Won, S.-Y. Choi, and S.M. Oh, Activation of TOPK by lipopolysaccharide promotes induction of inducible nitric oxide synthase through NF-kB activity in leukemia cells. Cellular Signalling, 26(5). 849-856 (2014)
• ‣ Pyo, C.W. J.H. Choi, S.M. Oh, and S.-Y. Choi, Oxidative stress-induced cyclin D1 depletion and its role in cell cycle processing. Biochimica et Biophysica Acta, 1830(11), 5316-5325 (2013)
• ‣ Kim, C.W., K.N. Son, S.-Y. Choi and J. Kim, Human lactoferrin suppresses TNFα-induced Intercellular Adhesion Molecule-1 expression via competition with NF-kB in endothelial cells, FEBS Letters 586, 229-234 (2012)
• ‣ Kim, K.C., H.G. Kim, T.Y. Roh, J. Park, K.-M. Jung, J.S. Lee, S.-Y. Choi,, S.S., Kim, B.S. Choi, The effect of CD4 receptor downregulation and its downstream signaling molecules on HIV-1 latency. Biochem. Bioph. Res. Co., 404(2), 646-651 (2011)
• ‣ Yeom, M., J. Park. B. Lee. S.-Y. Choi. K.S. Kim. H. Lee. and D.H. Hahm, Lactoferrin inhibits the inflammatory and angiogenic activation of bovine aortic endothelial cells. Inflamm. Res., 60,475-482 (2011)
• ‣ Kim, H.G., K.C. Kim, T.-Y. Roh, J. Park, K.-M. Jung, J.-S. Lee, S.-Y. Choi, S.S. Kim and B.-S. Choi, Gene silencing in HIV-1 latency by polycomb repressive group. Virology Journal 8, 179 (2011)
• ‣ Oh, Y.T., K.C. Kim, K.J. Hong, H.S. Lee, D.H. Jang, J.S. Lee, S.-Y. Choi, S.S. Kim, B.S. Choi, Establishment of novel cell lines latently infected with human immunodeficiency virus 1. Acta Virol. 55(2):155-159 (2011)
• ‣ Lee, H.S., K.Y. Lee, S.-Y. Choi, S.Y. Lee, Scale-up on mycelial morphology during liquid culture of Ganoderma lucidum, KSBB J. 26, 357-364 (2011)
• ‣ Kim, E.J., J. Kwon, S.H. Park, C. Park, Y.B. Seo, H.K. Shin, H.K. Kim, K.S. Lee, S.-Y. Choi, D.H. Ryu, G.S. Hwang, Metabolite profiling of Angelica gigas from different geographical origins using 1H NMR and UPLC-MS analyses. J. Agr. Food Chem. 59(16):8806-15 (2011)
• ‣ Lee, S.H., H.M. Hwang, C.W. Pyo, D.H. Hahm, and S.-Y. Choi, E2F1-directed Activation of Bcl-2 Is Correlated with Lactoferrin-Induced Apoptosis in Jurkat Leukemia T Lymphocytes, Biometals, 23(3), 507-514 (2010)
• ‣ Choi, H.M., Y.A. Lee, S.H. Lee, S.J. Hong, D.H. Hahm, S.-Y. Choi, H.I. Yang, M.C. Yoo and K.S. Kim, Adiponectin may contribute to synovitis and joint destruction in rheumatoid arthritis by stimulating vascular endothelial growth factor, matrix metalloproteinase-1, and matrix metalloproteinase-13 expression in fibroblast-like synoviocytes more than proinflammatory mediators, Arthritis Res. Ther., 11 (6), R161 (2009)
• ‣ Kim, S.A., J.H. Baek, S.J. Lee, S.-Y. Choi, W. Hur, and S.Y. Lee, A novel method for air drying aloe leaf slices by covering with filter papers as a shrink-proof layer, J. Food Sci., 74(9), 462-470 (2009)
• ‣ Lee, S.H., C.W. Pyo, D.H. Hahm, J. Kim and S.-Y. Choi, Iron-saturated lactoferrin stimulates cell cycle progression through PI3K/Akt pathway, Mol. Cells, 28, 37-42 (2009)
• ‣ Kang, J.I., S.N. Kwon, S.H. Park, Y.K. Kim, S.-Y. Choi, J.P. Kim and B.Y. Ahn, PKR protein kinase is activated by hepatitis C virus and inhibits viral replication through translational control. Virus Research, 142, 51-56 (2009)
• ‣ Lee, S.H., S.W. Park, C.W. Pyo, N.K. Yoo, J. Kim and S.-Y. Choi, Requirement of JNK-associated Bcl-2 pathway for human lactoferrin-induced apoptosis in Jurkat leukemia T cell line. Biochimie, 91, 102-108 (2009)
• ‣ Pyo, C.W., S.H. Lee, and S.-Y. Choi, Oxidative Stress Induces PKR-Dependent Apoptosis via IFN-gamma Activation Signaling in Jurkat T cells. Biochem. Bioph. Res. Co., 377, 1001-1006 (2008)
• ‣ Pyo, C.W., Y.L. Yang, N.K. Yoo, and S.-Y. Choi, Reactive oxygen species activate HIV long terminal repeat via post-translational control of NF-kB. Biochem. Bioph. Res. Co., 376, 180-185 (2008)
• ‣ Park, J.Y., H.W. Yoo, B.R. Kim, R. Park, S.-Y. Choi* and Y. Kim*(co-correspondence), Identification of a novel human Rad51 variant that promotes DNA strand exchange with prominent expression in the testis. Nucleic Acids Res. 36(10) 3226-3234) (2008)
• ‣ Yoo, N.K., C.W. Pyo, Y. Kim, B.Y. Ahn, and S.-Y. Choi, Vaccinia virus-mediated cell cycle alteration involves inactivation of tumor suppressors associated with Brf1 and TBP. Cell. Microbiol. 10(3), 583-592) (2008)
• ‣ Oh, S.M., S.H. Lee, B.J. Lee, N.K. Yoo, C.W. Pyo, S.Y. Lee, J. Kim, and S.-Y. Choi, A distinct role of neutrophil lactoferrin in RelA/p65 phosphorylation on Ser536 by recruiting TRAFs to IKK signaling complex. J. Immunol., 179(9), 5686-5692 (2007)
• ‣ Park, S.W. and S.-Y. Choi, Long-term expression of von Willebrand factor by VSV-G pseudotyped lentivirus to aid functional activity of secreted B-domain deleted coagulation factor VIII. Mol. Cells., 24(1), 125-131 (2007)
• ‣ Yang, H.M., H.J. Do, D.K. Kim, J.K Park, W.K Chang, H.M., Chung, S.-Y. Choi*, J.H. Kim*(co-correspondence), Transcriptional regulation of human Oct4 by steroidogenic factor-1. J. Cell. Biochem., 101(5), 1198-1209 (2007)
• ‣ Kim, C.W., K.N. Son, S. Choi and J. Kim, Human lactoferrin upregulates expression of KDR/Flk-1 and stimulates VEGF-A-mediated endothelial cell proliferation and migration. FEBS Lett. 580, 4332-4336 (2006)
• ‣ Park, S.H., J.D. Choi, J.I. Kang, S.-Y. Choi, S.B. Hwang, J.S. Kim, and B.Y. Ahn, Attenuated expression of interferon-induced protein kinase PKR in a simian cell devoid of type I interferons. Mol. Cells. 21, 21-28 (2006)
• ‣ Son, H.J., S.H. Lee. and S.-Y. Choi, Human lactoferrin controls the level of retinoblastoma protein and its activity. Biochem. Cell Biol. 84: 345-350 (2006)
• ‣ Kim, D.H., K.H. Yoo, Y.S. Yim, J. Choi, H.L. Jung, K.W. Sung, K.S. S.E. Yang, W.I. Oh, Choi, Y.S. Yang, S.-Y. Choi, and H.H. Koo, Cotransplanted bone marrow-derived mesenchymal stem cell enhanced engraftment of hematopoietic stem cells in MSC-dose dependent manner in NOD/SCID mice. J. Kor. Med. Sci. 21, 1000-1004 (2006)
• ‣ Lee, S.H., J.I. Yang, S.M. Hong, D.H. Hahm, S.Y. Lee, I.H. Kim and S.-Y. Choi, Phosphorylation of Peptides Derived from Isolated Soybean Protein: Effects on Calcium Binding, Solubility and Influx into Caco-2 Cells. Biofactors 23(3), 121-128 (2005)
• ‣ Yang, H.M., Do, H.-J., Oh, J.H., Kim, J.H., Choi, S.-Y., Cha, K.Y., Chung, H.M., Kim, J.H., Characterization of putative cis-regulatory elements that control the transcriptional activity of the human Oct4 promoter. J. Cell. Biochem. 96(4), 821-830 (2005)
• ‣ Kim, J.H., S.K. Choi, S.-Y. Choi, H.K. Kim, and H.I. Chang, Suppressive effects of Astaxanthin isolated from the Xanthophyllomyces dendrorhous mutant on ethanol-induced gastric mucosal injury in rats. Biosci. Biotech. Biochem. 69(7), 1300-1305 (2005)
• ‣ Kim, D.H., K.H. Yoo, K.S. Choi, J. Choi, S.-Y. Choi, S.-E. Yang, Y.S. Yang, H.L. Jung, K.W. Sung and H.H. Koo, Gene expression profile of cytokine and growth factor during differentiation of bone marrow-derived mesenchymal stem cell. Cytokine 31, 119-126 (2005)
• ‣ Park, J.W., S.-Y. Choi, H.J. Hwang, and Y.-B. Kim, Fungal mycoflora and mycotoxins in Korean polished rice destined for humans. Int. J. Food Micro. 103(3), 305-314 (2005)
• ‣ Kim, H.H., K.S. Ahn, H. Han, S.Y. Choung, S.-Y. Choi, and I.H. Kim, Decursin and PDBu: two PKC activators distinctively acting in the megakaryocytic differentiation of K562 human erythroleukemia cells. Leuk. Res. 29(12):1407-1413 (2005)
• ‣ Yang, H.N., J.G. Nam, S.S. Kim, S.-Y. Choi, and J.S. Lee, Analysis on quasispecies of HIV-1 env gene by single clone per PCR. J. Bacteriol. Virol. 35:2 134-140 (2005)
• ‣ Oh, S.M., C.W. Pyo, Y. Kim, and S.-Y. Choi, Neutrophil lactoferrin upregulates the human p53 gene through induction of NF-kB activation cascade. Oncogene 23, 8282-8291 (2004)
• ‣ Yang, J.I, S.H. Lee, D.H. Hahm, I.H. Kim and S.-Y. Choi, Enhancement of Calcium Binding Quality of Proglycinin Peptides by Chemical Phosphorylation. J. Microbiol. Biotechnol. 14:3, 607-611 (2004)
• ‣ Park, S.W. and S.-Y. Choi, Stable Gene transfer system by pseudotyped retrovirus vectors in hematopoietic progenitor cells from human bone marrow. Mol. Cells. 17, 297-230 (2004)
• ‣ Kim, M.S. S.S. Kim, S.T. Jung, J.Y. Park, H.W. Yoo, J. Ko, K. Csiszar, S.-Y. Choi, and Y. Kim, Expression and purification of enzymatically active forms of the human lysyloxidase-like protein 4. J. Biol. Chem. 278: 52071-52074 (2003)
• ‣ Choi, S.-Y.*(correspondence), H.J. Kim, J.I. Yang, and H.I. Chang, Translation initiation factor IF1-dependent stimulation of 30 S preinitiation complex formation: Rapid isolation and fMet-tRNA binding activity of IF1. J. Microbiol. Biotech. 11(6), 986-993 (2001)
• ‣ Oh, S.M., D.H. Hahm, I.H. Kim, and S.-Y. Choi, Human neutrophil lactoferrin trans-activates the matrix metalloproteinase 1 gene through stress-activated MAPK signaling modules. J. Biol. Chem. 276:45, 42575-42579 (2001)
• ‣ Park, H.J., S.H. Kim, S.T. Lim, D.H. Shin, S.-Y. Choi, and K.T. Hwang, Grease resistance and mechanical properties of isolated soy protein coated paper. J. Amer. Oil Chem. Soc. 77(3): 273-277 (2000)
• ‣ Kim, M.R., S.-Y. Choi*(correspondence), C.S. Kim, C.W. Kim, S. Utsumi, and C.H. Lee, Amino acid sequence analysis of bitter peptides from a soybean proglycinin subunit synthesized in E. coli. Biosci. Biotech. Biochem. 63:12 2069-2074 (1999)
• ‣ Kim, M.R., S.-Y. Choi, and C.H. Lee, Molecular characterization and bitter taste formation of tryptic hydrolysis of 11S glycinin. J. Microbiol. Biotech. 9:4 509-513 (1999)
• ‣ Jang, J.E., S.-Y. Choi, Y.H. Ko, K.H. Kim, K.S. Ahn, C.H. Park, J.H. Yang, S.J. Nam, and S.S. Yoon, Evaluation of VEGF expression in breast cancer. J. Kor. Can. Assoc. 31:3 483-491 (1999)
• ‣ Min, K.H., Y.C. Sung, S.-Y. Choi, and B.Y. Ahn, Functional interactions between conserved motifs of hepatitis C virus RNA helicase NS3 protein. Virus Genes 19:1 33-43 (1999)
• ‣ Faller, D.V., H. Weng, D.T. Graves, and S.-Y. Choi, Moloney murine leukemia virus activates monocyte chemotactic protein-1 protein expression and chemotactic activity. J. Cell. Physiol. 172 (2) 240-252 (1997)
• ‣ Faller, D.V., H. Weng, and S.-Y. Choi, Activation of collagenase IV gene expression and enzymatic activity by the Moloney murine leukemia long terminal repeat. Virology 227 (2): 331-342 (1997)
• ‣ Choi, S.-Y., K. Van de Mark, and D.V. Faller, Identification of cis-acting element in the class I Major Histocompatibility Complex gene promoter responsive to activation by retroviral sequences. J. Virol. 71: 965-970 (1997)
• ‣ Weng, H., S.-Y. Choi, and D.V. Faller, The Moloney retroviral long terminal repeat trans-activates AP-1-inducible genes and AP-1 transcription factor binding. J. Biol. Chem. 270: 13637-13644 (1995)
• ‣ Choi, S.-Y. and D.V. Faller, A transcript from the long terminal repeats of a murine retrovirus associated with trans-activation of cellular genes. J. Virol. 69: 7054-7060 (1995)
• ‣ Choi, S.-Y. and D.V. Faller (1994) The long terminal repeats of a murine retrovirus encode a trans-activator for cellular genes. J. Biol. Chem. 269: 19691-19694
• ‣ Murtha-Riel, P, M.V. Davies, B.J. Scherer, S.-Y. Choi, J.W.B. Hershey, and R.J. Kaufman, Expression of a phosphorylation-resistant eukaryotic initiation factor 2 α-subunit mitigates heat shock inhibition of protein synthesis. J. Biol. Chem. 268: 12946-12951 (1993)
• ‣ Choi, S.-Y., J. Scherer, J. Schnier, M.V. Davies, R.J. Kaufman, and J.W.B. Hershey, Stimulation of protein synthesis in COS cells transfected with variants of the α-subunit of initiation factor eIF-2, J. Biol. Chem. 267: 286-292 (1992)

 The specific goals of our laboratory are to establish an interdisciplinary approach to investigate the fundamental control mechanisms of gene expression process in conjunction with viral infection and stress-related disorders. Current efforts focus on the investigation of the biological roles of cellular and viral gene factors and their impacts on cellular defense mechanism. One of these studies has been extended to the establishment of a gene therapy technique in bone marrow or primary cells. These works will be thus allowing us to provide a ultimate therapy scheme for the treatment of a variety of cellular disorder and deficiency.

Research Area

• ‣ Transcriptional and translational control of cellular and viral genes
• ‣ Control mechanism in degenerative diseases
• ‣ Physiologically functional molecules and their roles in cell metabolism

Ongoing Projects

• ‣ Control of gene expression in response to virus infection and ER stress
• ‣ Biomedical approach to therapeutic defenses in degenerative diseases
• ‣ Lentivirus-based regulated-secretion system for the treatment of haemophilia A

(Under construction)

(Under construction)