• 로그인
QUICK MENU

고려대학교 교수소개

Knowledge & Innovation

소개

Prof. 고영규

Tel:  02-3290-3453 (office)

Tel:  02-3290-3961 (Lab.)

E-mail: ygko@korea.ac.kr

  • About Professor
  • Curriculum Vitae
  • Publication
  • Research
  • Teaching
  • Lab Members
  • Profile

     Prof. Young-Gyu, Ko. The Director of the research center of Tunneling Nano Tubes 

     

    Discovery of Extracellular Nanotubes

    ▶ For the first time, We discoverd innumerable fine tubes which connect cells each other in extracellular space of mouse primary hepatocyte ex vivo by confocal microscope. These tubes are known 'Tunneling Nano Tubes(TNT)' and they basically consist of actin filament and lipid raft.

     

     

    4 key questions for TNT research

    ▶ Although It is about a decade after TNTs were found in mammalian cells, those 4 core questions have not been solved yet. We have been challenging them. 

     

    • • How TNTs are conformed?
    • • What proteins compose TNTs?
    • • What is the function of TNTs?
    • • Is that possible to use TNTs for medical application?
    •  

    The research center of Tunneling Nano Tubes 

    ▶ For solving thoes questions, We organized the research center of Tunneling Nano Tubes with other 10 professors in division of Life Sciences, Korea University.

     

     

  • Curriculum Vitae

    Education
    • 박사 (1989-1994): 오스틴 소재 텍사스 주립대학 생명과학과
    • 석사 (1985-1987): 고려대학교 생물학과
    • 학사 (1981-1985): 고려대학교 생물학과
    Professional Experiences
    • 2016-현재 : 고려대학교 터널링나노튜브 연구센터 소장
    • 2008-현재 : 고려대학교 생명과학부 교수
    • 2005- 2008: 고려대학교 생명과학부 부교수
    • 2002- 2008: 고려대학교 생명과학부 조교수
    • 1999- 2002: 서울대학교 약학대학 창의연구단 책임연구원
    • 1996- 1999: 서울대학교 의과대학 암연구소 박사후 과정
    • 1994- 1996: Univ. of Texas Southwestern Medical School, 박사후 과정
    수상 경력
    • 석탑강의상 6 회 수상 (2003, 2009, 2010, 2011, 2012, 2013 년 )
    • 우수강의상 3 회 수상 (2010, 2011, 2012 년 )
    • 명강의상 1 회 수상 ( 교육대학원 , 2014 년 )
  • Publication

     

    Year 2020

    1. Lee, Hyun., Yu Dong‐Min., Park, Jun Sub., Hwayeon, Lee., Kim, Jun‐Seok., Kim, Hong Lim., Koo, Seung‐Hoi., Jae‐Seon, Lee., Lee, Sungsoo., Ko, Young‐Gyu. (2020) Prominin‐1‐Radixin axis controls hepatic gluconeogenesis by regulating PKA activity. EMBO Reports. doi: 10.15252 
     
    2. Lee, Hyung Chul., Kang, Donghee., Han, Namshik ... Ko, Young-Gyu., Gorospe, Myriam., Lee, Jae-Seon. (2020) A novel long noncoding RNA Linc-ASEN represses cellular senescence through multileveled reduction of p21 expression. Cell Death & Differentiation. 27(6):1844-1861.
     
    3. Kang, Donghee., Jung, Seung Hee., Lee, Gun-Hee., Lee, Seongju., Park, Heon Joo.,  Ko, Young-Gyu., Kim, Yong-Nyun., Lee, Jae-Seon. (2020) Sulfated syndecan 1 is critical to preventing cellular senescence by modulating fibroblast growth factor receptor endocytosis. FASEB Journal. doi: 10.1096.
     
     
    Year 2019
    1. Jung, Seung Hee., Lee, Minyoung., Park, Hyun A., Lee, Hyung Chul., Kang, Donghee., Hwang, Hyun Jung., Park, Chanho., Yu, Dong-Min ... Ko, Young-Gyu., Lee, Jae-Seon. (2019) Integrin alpha 6 beta 4-Src-AKT signaling induces cellular senescence by counteracting apoptosis in irradiated tumor cells and tissues. Cell Death & Differentiation. 26(2):245-259. 
     
    2. Jung, Seung Hee., Hwang, Hyun Jung., Kang, Donghee., Park, Hyun A., Lee, Hyung Chul.,  Jeong, Daecheol., Lee, Keunwook., Park, Heon Joo., Ko, Young-Gyu., Lee, Jae-Seon. (2019)  mTOR kinase leads to PTEN-loss-induced cellular senescence by phosphorylating p53.  Oncogene. 38(10):1639-1650.
     
    3. Shin, Kyungshin., Koo, Kyung Hee., Jeong, Jaemin., Park, Sang Jun., Choi, Dong Jin., Ko, Young-Gyu., Kwon, Heechung. (2019) Three-Dimensional Culture of Salivary Gland Stem Cell in Orthotropic Decellularized Extracellular Matrix Hydrogels. Tissue Engineering Part A. 25(19-20):1396-1403.
     
    4. Kim, Bong-Woo., Lee, Jae-Seon., Ko, Young-Gyu. (2019) Mycoplasma exploits mammalian tunneling nanotubes for cell-to-cell dissemination. BMB Reports. 52(8):490-495.
     
    Year 2018
    1. Park, Jun Sub., Lee, Hyun., Choi, Bo Woon., Ro, Seonggu., Lee, Doyoung., Na, Jeong Eun., Hong, Jeoung-Ho., Lee, Jae-Seon., Kim, Bong-Woo., Ko, Young-Gyu. (2018) An MG53-IRS1-interaction disruptor ameliorates insulin resistance. Experimental & Molecular Medicine. 50(6):1-12.
     
    2. Choi, Mi-Hyun., Na, Ji Eun., Yoon, Ye Ran., Rhyu, Im Joo., Ko, Young-Gyu., Baik, Ja-Hyun. (2018) Hypomyelination and cognitive impairment in mice lacking CD133 (Prominin-1). Biochemical and Biophysical Research Communications. 502(3):291-298.
     
    3. Shin, Kyungshin., Ko, Young-Gyu., Jeong, Jaemin., Kwon, Heechung. (2018) Fbxw7 beta is an inducing mediator of dexamethasone-induced skeletal muscle atrophy in vivo with the axis of Fbxw7 beta-myogenin-atrogenes. Molecular Biology Reports. 45(4):625-631.
     
    4. Yoon, Ye Ran., Lee, Tae-Gul., Choi, Mi-Hyun., Shin, Seung Woo., Ko, Young-Gyu., Rhyu, Im Joo., Kim, Dong-Hoon., Seong, Je Kyung., Baik, Ja-Hyun., (2018) Glucose-regulated protein 78 binds to and regulates the melanocortin-4 receptor. Experimental & Molecular Medicine. 50(9):120
     
    Year 2017
    1. Shin, K., Ko, YG and Jeong J (2017) Skeletal muscle atrophy is induced by Fbxw beta via atrogene upregulation. Cell Biology International. 41, 213-220.
     
    2. Lee, HC., Her, NG., …. Ko, YG, and Lee, JS (2017) Radiation-inducible miR-770-5p sensitizes tumors to radiation through direct targeting of PDZ binding kinase. Cell Death Dis., 8, e2693.
     
    3. Shin, Kyungshin., Hwang, Sang-Gu., Choi, Ik Joon Ko., Young-Gyu., Jeong, Jaemin., Kwon, Heechung. (2017) Fbxw7 beta, E3 ubiquitin ligase, negative regulation of primary myoblast differentiation, proliferation and migration. Anim. Sci. J., 88, 712-719.
     
    4. Jung, SH., Lee HC., Hwang HJ., … Ko, YG., Park, HJ., and Lee, JS (2017) Acyl-CoA thioesterase 7 is involved in cell cycle progression via regulation of PKCz-p53-p21 signaling pathway. Cell Death Dis., 8(5):e2793.
     
    5. Lee, HC., Jung, SH., …. Ko, YG., Gorospe, M., and Lee, JS. (2017) WIG1 is crucial for AGO2-mediated ACOT7 mRNA silencing via miRNA-dependent and -independent mechanisms. Nucleic Acids Res., 45, 6894-6910.
     
    6. Yu, D.-M., Jung, S. H., An, H.-T., Hong, J., …., and Ko, Y.-G. (2017) Caveolin-1 deficiency induces premature senescence with mitochondrial dysfunction. Aging Cell., 16, 773-784.
     
    7. Lee, H., Kim, BW., Lee, JW., Hong, J., Lee, JW., Kim HL., Lee, JS., and Ko, YG. (2017) Extracellular reactive oxygen species are generated by a plasma membrane oxidative phosphorylation system. Free Radic. Biol. Med., 112, 504-514. 
     

    Year 2016

    • 1. Hwang, H.-J., Jung, S. H., Lee, H. C., Han, N. K., Bae, I. H., Lee, M., Han, Y.-H., Kang, Y.-S., Lee, S.-J., Park, H. J., Ko, Y.-G., and Lee, J.-S. (2016) Idenfication of novel therapeutic targets in the secretome of ionizing radiation-induced senescent tumor cells. Oncology Reports. 35. 841-850.

    • 2. Lee, H., Kim, S.-H., Lee, J.-S., Yang, Y.-H., Nam, J.-M., Lee, J.-S., Kim, B.-W., and Ko, Y.-G. (2016) Mitochondrial oxidative phosphorylation complexes exist in the sarcolemma of skeletal muscle. BMB Reports, 49, 116-121.

    • 3. Jung, SH, Lee, HC, Yu, DM., Kim, BC, Park, SM, Lee, YS, Park, HJ, Ko, Y-G, and Lee, J-S (2016) Haparan sulfation is essential for the prevention of cellular senescence. Cell Death Differ., 23, 417-429.

    • 4 . Hong, J., Park, J.-S., Lee, H., Heong, J.-M., Yun, H. H., Ko, Y.-G * ., and Lee, J.-W * . (2016) Myosin heavy chain is stabilized by BCL-2 interacting cell death suppressor (BIS) in skeletal muscle. Exp. Mol. Med., 48:e225. * co-corresponding author

    • 5. Tan, T., Ko, Y.-G * ., and Ma, J * . (2016) Dual function of MG53 in membrane repair and insulin signaling. BMB Reports. 49(8):414-23 * co-corresponding author

    • 6. Kim, B.-C., Hwang, H.-J., Lee, H., Park, J.-S., Ahn, H. T., Ko, J., Lee, J.-S., and Ko, Y.-G. (2016) Antibody neutralization of cell-surface gC1qR/HABP1/SF2-p32 prevents lamellipodia formation and tumorigenesis. Oncotarget. 2;7(31):49972-49985.

    Year 2015

    • 1 . Jeong, Y.-J., And Jung, M.-G., Son, Y., Jang, J. H., Kim, S. H., Ko, Y.-G., Lee, Y.-S., and Lee, H. J. (2015) Coniferyl aldehyde attenuates radiation enteropathy by inhibiting cell death and promoting endothelial cell function. PLos One, e0128552.

    • 2. Lee, H.-C., Hong, M.-N., Jung, S. H., Kim, B. C., Suh, Y. J., Ko, Y.-G., Lee, Y.-S., Lee, B.-Y., Cho, Y.-G., Myung, S.-H., and Lee, J.-S. (2015) Effect of extremely low frequency magnetic fields on cell proliferation and gene expression. Bioelectromagnetics. 36, 506-516.

    Year 2014

    • 1 . Hong, J., Kim, B.-W., Choo, H.-J., Park, J.-J., Yu, D.-M., Lee, H., Yoon, G., Lee, J.-S., and Ko, Y.-G. (2014) Mitochondrial complex I deficiency enhances skeletal myogenesis but impairs insulin signaling through SIRT1 inactivation. J. Biol. Chem., 289, 20012-20025. “Recommended in Faculty of 1000Prime”. 8 citations.

    • 2 . Kim, B.-C., Yoo, H.-J., Lee, H.-C., Kang, K.-A., Jung, S.-H., Lee, H.-J., Lee, M., Park, S., Yi, Y.-H., Lee, Y.-S., Ko, Y.-G.* , and Lee J.-S. (2014) Evaluation of premature senescence and senescence biomarkers in carcinoma cells and xenograft mice exposed to single or fractionated irradiation. Oncol. Rep. , 31, 2229-2235. * co-corresponding author

    • 3 . Nguyen, N., Yi, J.-S., Park, H., Lee, J.-S., and Ko, Y.-G. (2014) Mitsugumin 53 (MG5) ligase ubiquitinates focal adhesion kinase during skeletal myogenesis. J. Biol. Chem. , 289, 3209-3216. 7 citations.

    • 4 . Kang, K.-A., Lee, H.-C., Lee, J.-J., Hong, M.-N., Park, M.-J., Choi, H.-D., Kim, Na., Ko, Y.-G.* , and Lee, J.-S.* (2014) Effects of combined radiofrequency radiation exposure on levels of reactive oxygen species in neuronal cells. J. Radiat. Res. , 55, 265-276. * co-corresponding author


    Year 2013

    • 1. Yi, J.-S., Ham, Y.-M., Lee, N.-R., …, Lee, C.-S., Lee, J.-S., Ko, Y.-G. (2013) MG53-induced IRS-1 ubiquitination negatively regulates skeletal myogenesis and insulin signaling. Nat . Commun . 4:2354 doi: 10.1038/ncomms3354 . 27 citations

    • 2. Yi, J.-S., Moon, D.-K., Park, J.-S., Lee, H., Lee, J.-W., Lee, J.-S., Kim, S.-J., Cho, B.-R., Lee, S.-W.*, and Ko, Y.-G. * (2013) PTRF/Cavin-1 is essential for multidrug resistance in cancer cells. J. Proteome Res. , 12, 605-614. * co-corresponding author. 15 citations.

    • 3. Jung, K.-J., Rho, J.-K., Kim, Y.-M., Jung, J. E., Jin, Y. G., Ko, Y.-G., Lee, J.-S., Lee, J. C., Park, M.-J. (201 3 ) Upregulation of CXCR4 is functionally crucial for maintenance of stemness in drug-resistant non-small cell lung cancer cells. Oncogene , 32, 209-221. 78 citations.

    Year 2012

    • 1. Kim, B. C., Lee, H. C., Lee, J.-J., Choi, C.-M., Kim, D.-K., Lee, J. C., Ko, Y.-G., and Lee, J.-S. (2012) Wig1 prevents cellular senescence via regulation of p21 mRNA decay through control of RISC recruitment. EMBO J., 31, 4289-4303. 10 citations

    • 2. Park, J.-J., Yi, J. Y., Jin, Y. B., Lee, Y.-J., Lee, J.-S., Lee, Y.-S., Ko, Y.-G.* , and Lee, M.* (2012) Sialylation of epidermal growth factor receptor regulates receptor activity and chemosensitivity to gefitinib in colon cancer cells. Biochem. Parmacol. , 83, 849-857. * co-corresponding author. 38 citations.

    • 3. Han, N.-K., Kim, B.-C., Lee, H.-C., Lee, Y.-J., Park, M.-J., Chi, S.-G., Ko, Y.-G., and Lee, J.-S. (2012) Secretome analysis of ionizing radiation-induced senescent cancer cells reveals that secreted RKIP plays a critical role in neighboring cell migration. Proteomics, 12, 2822-2832. 12 citations.

    • 4. Park, JJ., Seo, SM., Kim, EJ, and Lee YJ, Ko YG, Ha J, and Lee M. (2012) Berberine inhibits human colon cancer cell migration via AMP-activated protein kinase-mediated downregulation of integrin b1 signaling. Biochem. Biophys. Res. Commun., 426, 461-467. 42 citations.

    • 5. Hong M.-N., Kim, B.-C., Ko, Y.-G., Lee, Y.-S., Hong, S.-C., Kim, T., Pack, J.-K., Choi, H.-D., Kim, N., and Lee, J.-S. (2012) Effects of 837 and 1950 MHz radiofrequency radiation exposure alone or com b ined on oxidative stress in MCF10A cells. Bioelectromagnetics . 33, 604-611 . 9 citations.

    • 6. Lee, M., Park, J.-J., Ko, Y.-G.* , and Lee, Y.-S.* (2012) Cleavage of ST6Gal I by radiation-induced BACE1 inhibits Gogi-anchored ST6Gal-mediated sialyation of integrin b1 and migration in colon cancer cells. Radiation Oncology , 7, 47. * co-corresponding author. 8 citations.

    • 7. Park, J.-J., Jin, Y. B., Lee, Y.-J., Lee, J.-S., Lee, Y.-S., Ko, Y.-G.* , and Lee, M.* (2012) KAI1 suppresses HIF-1a and VEGF expression by blocking CDCP1-enhanced Src activation in prostate cancer. BMC Cancer . 12, 81. * co-corresponding author. 14 citations

    • 8. Hong, M.-N., Kim, B.-C., Ko, Y.-K., Lee, Y.-S., Hong, S.-C., Kim, T., Pack, J.-K., Choi, H.-D., and Lee, J.-S. (2012) Effects of combined radiofrequency radiation exposure on the oxidative stress. Bioelectromagnetics . 53, 79-86. 9 citations.

    • 9. Kim, S., Seo, J., Ko, Y.-G., Huh, Y.-D., and Park, H. (2012). Lipid-binding properties of TRIM72. BMB Reports . 45, 26-31.


    Year 2011

    • 1. Kim, K.-B., Lee, J.-H., Yi, J.-S., Yoo, D.-M., Kwon, Y.-C., Ahn, B.-Y., Cho, H. Kim, Y.-K., Yoo, H.-J., Lee, J.-S., and Ko, Y.-G . , (2011) Cell surface gC1qR is a key regulator for lamellipodia formation and cancer metastasis. J. Biol. Chem. , 286, 23093-23101. “A paper of the week” . 25 citations

    • 2. Kim, J.-H., Kim, H., Yoon, Y.-S., Xu, W., Yoon, J.-K., Choi, S.-E., Ko, Y.-G. , Kim, M.-J., Lee, S.-J., Wang, H.-J., Yoon, G. (2011) Involvement of mitophage in o n cogenic K-Ras-induced transformation: overcoming a cellular energy deficit from glucose deficiency. Autophagy . 7, 1187-1198. 48 citations.

     

    Year 2010

    • 1. Lee, J.-J., Lee, J.-W., Ko, Y.-G . , Hong, S. I., and Lee, J.-S. (2010) Prevention of premature senescence requires JNK regulation of Bcl-2 and reactive oxygen species. Oncogene , 29, 561-575. 34 citations.

    • 2. Park, E.-Y., Kwon, O.-B., Joen, B. C., Lee, C.-S., Ko, Y.-G.* and Song, H.-K. * (2010) Crystal structure of PRY-SPRY domain of human TRIM72. Proteins (IF=2.813) . 78, 790-795. * co-corresponding author. 22 citations.

    • 3. Kim, K.-B., Byun, H.-O., Han, N.-K., Ko, Y.-G. , Choi, H.-D., Kim, N., Pack, J.- K., and Lee, J.-S. (2010) Two-dimensional electrophoretic analysis of radio frequency readiation-exposed MCF7 breast cancer cells. J. Radiol. Res. , 51, 205-213. 21 citations.

    • 4. Lee, C.-S., Ham, Y.-M., Kim, B.-W., Kwon, O.-B., Choo, H.-J., Jang, S.-Y., Chi, S.-G., Kim, J.-Y., Kim, J.-H., and Ko, Y.-G. (2010) TRIM72 negatively regulates myogenesis by targeting insulin receptor substrate-1. Cell Death Differ . , 17, 1254-1265. 35 citations.

    • 5. Jung, S.-Y., and Ko, Y.-G. (2010) TRIM72, a novel negative feedback regulator of myogenesis, is transcriptionally activated by the synergism of MyoD (or myogenin) and MEF2. Biochem. Biophys. Res. Commun. , 396, 238-245. 16 citations.

    • 6. Kim, B.-W., Lee, J.-W., Choo, H.-J., Lee, C.-S., Jung, S.-Y., Yi, J.-S. ..., and Ko, Y.-G. (2010) Mitochondrial oxidative phosphorylation system is recruited to detergent-resistant lipid rafts during myogenesis. Proteomics , 10, 2498-2515. “Cover paper” . 17 citations.

    • 7. Kim, J., Ahn, S., Ko, Y.-G. , Boo, Y.-C., Chi, S.-G., Ni, C.-W., Go, Y.-M., and Park, H. (2010) X-lined inhibitor of apoptosis protein controls integrin {alpha}5-mediated cell adhesion and migration. Am. J. Physiol. Hear Circ. Physiol. . 299, H300-309 . 18 citations.

    • 8. Kim, B.-W., Lee, C.-S., Yi, J.-S., Lee, J.-W., Choo, H.-J., Jung, S.-Y., ....., and Ko, Y.-G. (2010) Novel biological insights from lipid raft proteomics; the mitochondrial oxidative phosphorylation system is associated with detergent- resistant lipid rafts. Expert Rev. Proteomics , 7, 849-866 . 16 citations.

     

    Year 2009

    • 1. Kim, K.-B., Kim, B.-W., Choo, H.-J., Kwon, Y.-C., Ahn, B.-Y., Choi, J.-S., Lee, J.-S., and Ko, Y.-G. (2009) Proteome analysis of adipocyte lipid rafts reveals that gC1qR plays essential roles in adipogenesis and insulin signal transduction. Proteomics, 9, 2373-2382. “Selected in the issue” 31 citations

    • 2. Byun, H.-O., Han, N.-K., Lee, H.-J., Kim, K.-B., Ko, Y.-G. , Yoon, G., Lee, Y.-S., Hong, S.-I., and Lee, J.-S. (2009) Cathepsin D and eukaryotic translation elongation factor 1 as markers of stress-induced premature senescence. Cancer Res. , 69, 4638-4637 . 56 citations.

    • 3. Yi, J.-S., Choo, H.-J., Lee, C.-S., Kim, H.-M., Cho, B.-R., Ham, Y.-M., and Ko, Y.-G. (2009) Ginsenoside induces ligand-independent Fas activation via lipid raft disruption. Biochem. Biophys. Res. Commun. (IF=2.595) , 385, 154-159 . 39 citations.

    • 4. Kim, K. M., Cho, H., Choi, K., Kim, J., Kim, B.-W., Ko, Y.-G. , Jang S. K., and Kim, Y. K. (2009) A new MIF4G domain-containing protein PIROT directs the pioneer round of translation. Genes Develop. (IF=12.889), 23, 2033-2045 . 54 citations.

     

    Year 2008

    • 1. Kim, H.-M., Choo, H.-J., Ko, Y.-G. , Jeon, S.-J., Kim, C. H., Joo, T., and Cho, B.-R. (2008) Two-photon fluorescent probes for lipid raft imaging. Effect of chain length. ChemBioChem. 9, 2830-2838 . 19 citations.

    • 2. Lee, J. J., Kwak, H.-J., Lee, Y.-M., Park, M.-J., Ko, Y.-G. , Choi, H.-D., Kim, N., Pack, J.-K., Hong, S.-I., and Lee, J.-S. (2008) Radiofrequency radiation does not affect cell cycle, cellular migration, and invasion. Bioelectromagnetics, 29,615-625 . 13 citations.

    • 3. Lee, S.-J., Choi, D., Rhim, H., Choo, H.-J., Ko, Y.-G. , Kim, C.-G., and Kang S. (2008) PHB2 interacts with RNF2 and represses CP2c-stimulated transcription. Mol. Cell Biochem. , 319, 69-77. 9 citations.

    • 4. Choo, H.-J., Kim, B.-W., Kwon, O.-B., Lee, C.-S., Choi, J.-S. and Ko, Y.-G. (2008) Secretion of adenylate kinase 1 is required for extracellular ATP synthesis in C2C12 myotubes. Exp. Mol. Med. , 40, 220-228. 11 citations.

    • 5. Kim, K.-B., and Ko, Y.-G. (2008) Isolation of detergent-resistant lipid rafts for proteomic analysis. Methods Mol. Biol. 424, 413-422. 24 citations.


    Year 2007

    • 1. Kim, H.-M., Fang, X. Z., Yang, P. R., Yi, J.-S., Ko, Y.-G. , Piao, M. J., Chung, Y. D., Park, Y. W., Jeon, S.-J., and Cho, B.-R. (2007) Design of molecular two-photon probes for in vivo imaging 2H-Benzo [h]chromene-2-one derivatives. Tetrahedron Lett. 48, 2791-2795 . 44 citations.

    • 2. Kim, H.-B., Yang, P. R., Seo, M. S., Yi, J.-S., Hong, J. H., Jeon, S.-J., Ko, Y.-G. , Lee, K. J., and Cho, B. R. (2007) Magnesium ion selective two-photon fluorescent probe based on a Benzo[h] chromene derivative for in vivo imaging. J. Org. Chem. , 72, 2088-2096 . 100 citations.

    • 3. Kim, H.-M., Choo, H.-J., Jung, S.-Y., Ko, Y.-G. , Park, W.-H., Jeon, S.-J., Kim, C. H., Joo, T., and Cho, B. R. (2007) A two-photon fluorescent probe for lipid raft imaging: C-laurdan. ChemBioChem, 8, 553-559. 133 citations.

    • 4. Kim, H. M., Jung, C., Kim, B. R., Jung, S.-J., Hong, J. H., Ko, Y.-G. , Lee, K. J., and Cho, B. R. (2007) Environment-sensitive two-photon probe for intracellular free magnesium ions in live tissue. Angew. Chem. Int. Ed. , 46, 1-5 . 99 citations.

    • 5. Jin, J.-K., Ahn, B.-H., Na, Y.-J. Kim, J.-I., Kim, Y.-S., Choi, E.-K., Ko, Y.-G. , Chung K. C., Kozlowski, P. B., and Min, D. S. (2007) Phospholipase D1 is associated with amyloid precursor protein in Alzheimer’s disease. Neruobiol. Aging , 28, 1015-1027 . 29 citations.

    Year 2006

    • 1. Park, S. G., Kang, Y. S., Kim, J. Y., Lee C. S., Ko, Y.-G. , Lee, W. J., Lee, K.-U., Yeon, Y. I., and Kim, S. (2006) Hormonal activity of AIMP/p43 for glucose homeostasis. Proc. Nat’l. Acad. Sci. U. S. A . , 103, 14913-14918 . 49 citations.

    • 2. Kim, S.-I., Yi, J. S., and Ko, Y.-G. (2006). Amyloid b oligomerization is induced by brain lipid rafts. J. Cell. Biochem., 99, 878-889. 75 citations

    • 3. Choo, H.-J., Kim, J.-H., Kwon, O.-B., Lee, C. S., Lee, J. Y., Han, S. S., Youn, Y.-S., Yoon G., Choi, K.-M., and Ko, Y.-G. (2006) Mitochondria are impaired in the adipocytes of type 2 diabetic mice. Diabeteologia. 49, 784-791. “Introduced in commentary section” . 216 citations.

    • 4. Kim, K.-B., Lee, J.-W., Jung, S.-Y., Chi, S.-G., and Ko, Y.-G. (2006) Oxidation-reduction respiratory chains and ATP synthase complex are localized in the lipid rafts originating from plasma membrane. Proteomics , 6, 2444-2453. “Cover paper” . 81 citations .

    • 5. Rhee K. H., Nam, K. H., Lee W. H., Ko, Y.-G. , Kim, E. E., and Hwang K.-Y. (2006) Expression, purification, and preliminary X-ray crystallographic analysis of the complex G(alphai3)-RGS5 from human with GDP/Mg2+/AlF4-. Protein Pept. Lett., 13, 945-949


    Year 2005

    • 1. Yi, J.-S., Kim, J.-H., Park, S. G., Kim, S., and Ko, Y.-G. (2005) Aminoacyl-tRNA synthetase-interacting multi-functional protein, p43, is imported to endothelial cells via lipid rafts. J. Cell. Biochem., 96, 1286-1295 . 8 citations.

    • 2. Park, Y.-K., Lee, J.-W., Ko, Y.-G. , Hong, S., and Park, S.-H. (2005) Lipid rafts are required for efficient signal transduction by CD1d. Biochem. Biophys. Res. Commun. , 327, 1143-54. 30 citations.


    Year 2004

    • 1. Kim, B.-W., Choo, H.-J., Lee, J.-W., Kim, J.-H., and Ko, Y,-G. (2004) Extracellular ATP is generated by ATP synthase complex in adipocyte lipid rafts. Exp. Mol. Med. , 36, 476-85. 117 citations

    • 2. Kim, K.-B., Kim, S.-I., Choo, H.-J., Kim, J.-H., and Ko, Y.-G. (2004) Two dimensional electrophoretic analysis reveals that lipid rafts are intact at physiological temperatures. Proteomics , 4, 3527-35. 35 citations.

    • 3. Bae, T.-J., Kim, M.-S., Kim, J.-W., Kim, B.-W., Choo, H.-J., Lee, J.-W., Kim, K.-B., Lee, C.-S., Kim, J.-H., Chang, S. Y., Kang, C.-Y., Lee, S.-W., and Ko, Y.-G. (2004) Lipid raft proteome reveals ATP synthase complex in the cell surface. Proteomics , 4, 3536-48. 1 57 citations


    Year 2003

    • 1. Han, J., Lee, C.-S., Yun, C.-Y., Lee, B.-H., Ko, Y.-G. , Kang, C.-S., Lee, S.-D., Hwang, J.-S., Kang, S.-W., and Kim, H.-R. (2003) Cloning and expression of male-specific protein (MSP) from the hemolymph of greater wax moth, Galleria mellonella L. Arch. Insect. Biochem. Physiol. 54, 110-120. 10 citations.


    Year 2002

    • 1. Park, H., Park, S. G., Kim, J., Ko, Y.-G. , and Kim, S. (2002) Signaling pathways fo TNF production induced by human aminoacyl-tRNA synthetase-associating factor, p43. Cytokine 20, 148-153 . 61 citations.

    • 2. Park, H.-S., Cho, S.-G., Kim, C.-K., Hwang, H.-S., Noh, K.-T., Kim, M.-S., Huh, S.-H., Kim, M.-J., Ryoo, K., Kim, E.-K., Kang, W.-J., Lee, J.-S., Seo, J.-S., Ko, Y.-G. , Kim, S., and Choi, E.-J. (2002) Heat shock protein hsp72 is a negative regulator of apoptosis signal-regulating kinase 1. Mol. Cell. Biol. 22, 7721-7730 . 162 citations.

    • 3. Park, S. G., Kang, Y.-S., Ahn, Y. H., Lee, S. H., Kim, K. R., Kim, K. W., Koh, G.-Y., Ko, Y.-G. ., and Kim, S. (2002) Dose-dependent biphasic activity of tRNA synthetase-associating factor, p43, in angiogenesis. J. Biol. Chem. 277, 45243-45248 . 134 citations.

    • 4. Ko, Y.-G. , Park, H., and Kim, S. (2002) Novel regulatory interactions and activities of mammalian tRNA synthetases . Proteomics 2, 1304-1310. 35 citations.

    • 5. Kim, J.-Y., Kang, Y.-S., Lee, J.-W., Kim, H-J., Ahn, Y. H., Park, H., Ko, Y.-G. , and Kim, S. (2002). p38 is essential for cellular stability of macromolecular tRNA synthease complex and viability of mice. Proc. Nat’l. Acad. Sci. U. S. A. 102, 7912-1916 . 99 citations.


    Year 2001

    • 1. Ko, Y.-G. , Kang, Y.-S., Park, H., Seol, W., Kim, J., Kim, T., Park, H.-S., Choi, E.-J. and Kim, S. (2001) Apoptosis signal-regulating kinase 1 controls the proapoptotic function of Daxx in the cytoplasm. J. Biol. Chem. 276, 39103-39106. 124 citations .

    • 2. Ko, Y.-G. , Park, H., Kim, T., Lee, J.-W., Park, S. G., Kim, J. E., Seol, W., Lee, W.-H., Park, J.-E., and Kim, S. (2001) A cofactor of tRNA synthetase is released to upregulate proinflammatory chemokines and enriched in atherosclerosis lesions. J. Biol. Chem. 276, 23028-23033. 134 citations .

    • 3. Ko, Y.-G. Kim, E.-K., Park, H., Kim, T, Park, H.-S., Choi, E.-J., and Kim, S. (2001) Glutamine-dependent antiapoptotic interaction of human glutaminyl-tRNA synthetase with apoptosis signal-regulating kinase 1. J. Biol. Chem . 276, 6030-6036. 139 citations

     

    Year 2000

    • 1. Kang, J.-W., Kim, T., Ko, Y.-G., Rho, S. B., Park, S. G., Kim, M. J., Kwon, H. J., and Kim, S. (2000) Heat shock protein 90 mediates protein-protein interactions between human aminoacyl-tRNA synthetases. J. Biol. Chem. 275, 31682-31688. 36 citations.

    • 2. Lee, C.-Y., Lee, J.-S., Ko, Y.-G., J.-I. Kim, and Seo, J.-S. (2000) Hsp70 inhibits apoptosis downstream of cytochrome c release and upstream of caspase-3 activation. J. Biol. Chem. 275, 25665-25671. 460 citations.

    • 3. Kim, T., Park, S. G., Kim, J. E., Seol, W., Ko Y.-G. * , and Kim, S. (2000) Catalytic peptide of human glutaminyl-tRNA synthetase is essential for its assembly to the aminoacyl-tRNA synthetase complex. J. Biol. Chem. 275, 21768-21772. * Corresponding author . 39 citations.

    • 4. Park, W.-Y., Park, J.-S., Cho, K.-A., Kim, D.-I., Ko, Y.-G., Seo, J.-S., and Park, S.-C. (2000) Up-regulation of caveolin attenuates EGF signaling in senescent cells. J. Biol. Chem. 275, 20847-20852. 189 citations.

    • 5. Ko, Y.-G. , Kang, Y.-S., Kim, E.-K., Park, S.-K., and Kim, S. (2000) Nucleolar localization of human methionyl-tRNA synthetase and its role in ribosomal RNA synthesis. J. Cell Biol . 149 , 567-574. 87 citations .

    • 6. Lee, S. W., Ko, Y.-G., Bang, S. H., Kim, S., and Kim, K-S. (2000) Death effector domain of a mammalian apoptosis mediator, FADD, induces bacterial cell death. Mol. Microbiol . 35 , 1540-1549. 20 citations.

    • 7. Ko, Y.-G. , Kang, Y.-S., and Seo, J.-S. (2000) Caveolin-1 internalization by heat shock or hyperosmotice shock. Exp. Cell Res . 255 , 221-228. 36 citations.

     

    Year 1999-1988

    • 1. Ko, Y.-G. , Lee, J.-S., Kang, Y.-S., Ahn, J.-H., and Seo, J.-S. (1999) TNF-alpha-mediated apoptosis is initiated in caveolae-like domains. J. Immunol ., 162, 7217-7223 . 143 citations .

    • 2. Ahn J.-H., Ko, Y.-G., Park, W.-Y., Kang, Y.-S., Chung, H.-Y. and Seo, J.-S. (1999) Suppression of ceramide-mediated apoptosis by HSP70 . Mol. Cells 9 , 200-206 . 51 citations.

    • 3. Ko, Y.-G. , Liu, P., Pathak, R. K., Craig, L., and Anderson, R. G. W. (1998) Early effects of pp60v-src kinase activation on caveolae. J. Cell Biochem . 71 , 524-535. 41 citations.

    • 4. Liu, P., Ying, Y., Ko, Y.-G., and Anderson, R. G. W. (1996) Localization of platelet-derived growth factor-stimulated phosphorylation cascade to caveolae. J. Biol. Chem . 271 , 10299-10303. 391 citations.

    • 5. Thompson G. A. Jr., Morita, N., Okuyama, H., Kim, Y., Ko, Y.-G., and Hung, C.-Y. On the presence of glycosylphosphatidylinositol-anchored proteins in plants. Plant Lipid Metabol., 227-229, 1995. 1 citations.

    • 6. Ko, Y.-G. , Hung, C.-Y., and Thompson, G. A. Jr. (1995) Temperature regulation of the Tetrahymena mimbres glycosylphosphatidylinositol-anchored protein lipid composition. Biochem. J. 307, 115-121 . 14 citations.

    • 7. Hung, C.-Y., Ko, Y.-G. , Thompson, G. A. Jr. (1995) Temperature-induced alteration of inositolphosphorylceramides in the putative glycosylated lipid precursors of Tetrahymena mimbres glycosylphosphatidylinositol-anchored proteins. Biochem. J . , 307, 107-113. 10 citations.

    • 8. Ko, Y.-G. , and Thompson, G. A. Jr. (1995) Purification of glycosylphosphatidylinositol-anchored proteins by modified Triton X-114 partitioning and preparative gel electrophoresis. Anal. Biochem . 224, 166-172. 42 citations.

    • 9. Ko, Y.-G. , and Thompson, G. A. Jr. (1992) Immobilization antigens from Tetrahymena thermophila are glycosylphosphatidylinositol-linked proteins. J. Protozool . 39 , 719-723. 25 citations.

    • 10. Ko, Y.-G. , and Kim, H.-R. (1988) Purification, characterization and synthesis of vitellin from thecabbage butterfly, Pieris rapae L. Korean Biochem. J . 21 , 471-478.

    • 11. Yeo, J.-W., Ko, Y.-G., and Kim H.-R. (1988) Some characteristics of hemolymph yolk protein in Pieris rapae L. Korean J. Entomol . 18 , 27-34.

    • 12. Kim, H.-R., Ko, Y.-G., and Mayer (1988) Purificaton, characterization and synthesis of vitellin from the cabbage butterfly, Pieris rapae L. Arch. Insect Biochem. Physiol . 9 , 67-79. 13 citations.


    Patents

    • 1. Young-Gyu Ko, Jae-Sung Yi, Chang-Seok Lee (2013) Use of TRIM72 as a target for muscle and heart enhancer. European Patent. Patent No., EP 2 185 174 B1. Date of Patent: Mar. 16, 2016

    • 2. 고영규 (2014) MG53-UBE2H 상호작용을 이용한 제 2형 당뇨 치료제의 스크리닝 방법. 국내특허 제 10-1460737호. 등록일2014년 11월 5일

    • 3. Young-Gyu Ko, Jae-Sung Yi, Chang-Seok Lee (2013) Use of TRIM72 as a target for muscle and heart enhancer. United States Patent. Patent No., US 8,383,602 B2. Date of Patent: Feb. 26, 2013

    • 4. 고영규 (2013) 층상 위족 형성 및 암 전이 조절용 조성물. 국내특허 제 10-1282374호. 등록일2013년 6월 28일

    • 5. 고영규, 이재성, 이창석 (2013) 근육 및 심장 강화제의 타겟으로서 TRIM72의 용동. 국내특허 제 10-10483164호. 등록일2011년 7월 5일

    • 6. Sunghoon Kim and Young-Gyu Ko (2006) Method of inducing production of IL-8 or TNF by in vitro administration of an N-terminal polypeptide of p43. United States Patent. Patent No., US 7,037,505 B2. Date of Patent: May 2, 2006

  • Research

    Research Area
     

    1. Gluconeogenesis in Liver

     Prominin-1 (Prom1) is a major cell surface marker of cancer stem cells, but its physiological functions in the liver have not been elucidated. We analyzed the levels of mRNA transcripts in serum-starved primary Prom1+/+ and Prom1-/- mouse hepatocytes using RNA-sequencing (RNA-seq) data, and found that CREB target genes were down-regulated. This initial observation led us to determine that the Prom1 deficiency inhibited cAMP response element binding protein (CREB) activation and gluconeogenesis, but not cyclic AMP (cAMP) accumulation, in glucagon-, epinephrine-, or forskolin-treated liver tissues and primary hepatocytes, and mitigated glucagon-induced hyperglycemia. Because Prom1 interacted with radixin, the Prom1 deficiency prevented radixin from localizing to the plasma membrane. Moreover, systemic adenoviral knockdown of radixin inhibited CREB activation and gluconeogenesis in glucagon-treated liver tissues and primary hepatocytes, and mitigated glucagon-elicited hyperglycemia. Based on these results, we conclude that Prom1 regulates hepatic PKA signaling via radixin functioning as an A kinase-anchored protein (AKAP).

     

    2. Lipid raft and Tunneling Nano Tubes

     If receptors and signaling molecules are randomly scattered in plasma membrane a lot of signaling pathways are slow and  inefficient. Hence, there are lipid rafts to organize those signal molecules and are responsible for the center of signaling. We have identified a few novel proteins in lipid rafts related to diabetes, senescence, cancer and muscle disease. For these research, we used a bunch of  biological tools like adenovirus, siRNA and also genetically modified mouse. Especially, we figured out that insulin resistance was removed in musle of TRIM72/MG53 knock out mouse because the TRIM72/MG53 negatively regulated muscle differenciation inducing IRS-1 ubiquitination. 

     Recently, we found CD133, one of the ripid rafts proteins, are important to conform the Tunneling Nano Tubes(TNTs) and have been researching the biological role of them.

     

    3. Liver Regeneration 

     Liver Regeneration is one of the reseach areas which mechanism has not been exactly eucidated. There are two major types of liver cells. Hepatocyte, the parencymal cell of liver and cholangiocyte composing bile duct. It has been argued that which type of cell is responsible for regeneration in liver injury because a lot of researchers have showed different results. We have been researching the type of cell and the role of proteins in liver regeneration executing partial hepatectomy and drug treatment like CCl4, DDC and MCDE to genetically modified mouse.  

     

     

    Ongoing Project
    • • 선도연구센터 지원사업 터널링 나노튜브 연구센터 ( 센터장 , 고영규 ): 2015-2022, 13 억 / 년
    • • 인슐린 저항성 치료제로서 MG53-IRS-1 결합억제제의 개발 ( 연구책임자 , 고영규 ): 2014-2019, 5 억 / 년
  • Teaching

    • • 생리학I(전공과목): 석탑강의상 7회 수상 (2003, 2009, 2010, 2011, 2012, 2013, 2016년), 우수 강의상 1회 수상(2014)
    • • 생리학(교육대학원): 명강의상 1회 수상(2014년)
    • • 생명공학과 미래사회(핵심교양과목): 우수강의상 3회 수상 (2010, 2011, 201, 2014년)
    • • 일반생물학
  • Lab Members

    Current

    • • Dr. Lee, Sungsoo(연구교수, Ph. D. from URI.)
    • • Dr. Lee, Hyun(박사후 과정, Ph. D. from Korea Univ.)
    • • Dr. Yoo, Dong-Min(박사후 과정, Ph. D. from Korea Univ.)
    • • Ban, Myong-Seok(석박사 통합과정)
    • • Byun, Cho-Hyeun(석사 후 연구원)
    • • Kwon, Young-Jae(석사과정)
    • • Jo, Seong-Je(석박사 통합과정)
    • • Lee, Myoung-Woo(석사과정)
    •  
    • • Kim, Eun-Jeong(행정직원)


     

    Former

    • • Dr. Park, Jun Seob: 한미약품
    • • Kim, Ki-Tae(M. S.)
    • • Lee, Hwa-Yeon(M. S.): 한미약품
    • • Yoon,Seo-Yeon(M. S.): 신풍제약
    • • Eom,Min-Ji(M. S.): 셀트리온
    • • Dr. Kim, Bong-Woo
    • • Dr. Lee, Je-Jung
    • • Dr. Kim, Bum-Chan: Samsung Inc.
    • • Hong, Jin: Postdocter in Baylor University
    • • Dr. Jung-Jin Park: Research Professor in Chungbuk Nat’l Univ.
    • • Dr. Nga Nguyen: A professor in Vietnam
    • • Dr. Joong-Won Lee: Postdocter in Scripps Inistitute
    • • Dr. Hyo-Jung Choo: Postdocter in Emory Univ.
    • • Dr. Jae-Sung Yi: Postdoctor in Yale Univ.
    • • Dr. Young-Mi Ham: Postdoctor in Harvard Univ.
    • • Ji-Yoen Lee (M. S.): 농업진흥연구소 연구원
    • • Dr. Tae-Jung Bae: Postdocter in USA
    • • Jun-Woo Kim (M. S.): LG 생명과학 연구원
    • • Dr. Soon-Young Jang: Postdocter in Yonsei Univ. Medical School
    • • Chan-Woong Park (M. S.): 생명공학연구소 연구원
    • • Joo-Hyung Lee (M. S.): 박사후과정생, Baylor Univ.
    • • Na-Rae Lee (M. S.): 유한양행 연구원
    • • Kim, Seung-Hyub: 경희대 치과대 학생
    • • Hwang, Hyun-Jeong (M. S.): 박사과정, Inha Univ
    • • Lee, Jeong-Woo (M. S.): 변리사
    • • Choi, Bo-Woon