• 구조 단백질체학 연구실 (Lab. of Structural Proteomics)
• 생명과학관 서관 513호 (Lab: 522호) 
• Room 513, West Building, Life Sciences & Biotechnology, Korea Universtiy, 
• 145 Anamro Seungbukgu, Seoul 

Education

• BS. Department of Chemistry Education, Seoul National University (1985.03 - 1989.02)


• MS. Department of Chemistry, Seoul National University (1989.03 - 1991.02)


• Ph.D Department of Chemistry, Seoul National University (1991.03 - 1994.08)

Professional Experiences

• Department of Biochemistry, Medical Research Center, University of Alberta, Canada (1995.03 - 1997.04)


• Korea Institute of Sciences & Technology, Structural Biology Center (1997.05 - 1998.12)


• LG Chem. Biotech Institute (1999.01 - 2000. 08)


• CrystalGenomics, Inc (2000.09 - 2003.08)


• Korea Institute of Sciences & Technology, Biomedical Center (2003.09 - 2005.08)


• Division of Biotechnology, College of Life Sciences & Biotechnology, Korea University (2005.09 - Current)

2023

Development of carbon nanoparticles-based soluble solid-phase immune sensor for the quantitative diagnosis of inflammation.

2022

Optimization for Simultaneous Removal of Product/Process-Related Impurities of Peptide Fc-Fusion Protein Using Cation Exchange Chromatography.

Bovine clolstrum derived-exosomes prevent dextran sulfate sodium-induced intestinal colitis via suppression of inflammation and oxidative stress.

Han G, Cho H, Kim H, Jang Y, Jang H, Kim DE, Kim ES, Kim EH, Hwang KY, Kim K, Yang Y & Kim SH, Biomaterials Science. 2022 Mar 22, 10. 8, p. 2076-2087 12 p.

Nano-sized  drug delivery systems to potentiate the immune checkpoint blockade therapy.

Insights into the structure of mature streptavidin C1 from Streptaomyces cinnamonensis reveal the self-binding of the extension C-terminal peptide to biotin-binding sites.

   

Recent advances in exosome-based drug delivery for cancer therapy.

 

Leucine-sensing mechanism of leucyl-tRNA synthetase 1 for mTORC1 activation.

2020

Monothiol and dithiol glutaredoxin-1 from Clostridium oremlandii: identification of domain-swapped structures by NMR, X-ray crystallography and HDX mass spectrometry.

Lee K, Yeo KJ, Choi SH, Lee EH, Kim BK, Kim S, Cheong HK,  Lee WK,  Kim HY, Hwang E, Woo JR, Lee SJ, Hwang KY*. IUCrJ. 2020  Sep

 

In Situ One-Step Fluorescence Labeling Strategy of Exosomes via Bioorthogonal Click Chemistry for Real-Time Exosome Tracking In Vitro and In Vivo.

Song S, Shim MK, Lim S, Moon Y, Yang S, Kim J, Hong Y, Yoon HY, Kim IS, Hwang KY*, Kim K*. Bioconjug Chem. 2020 May 20;31(5):1562-1574.

 

Structural Basis for the Antibiotic Resistance of Eukaryotic Isoleucyl-tRNA Synthetase.

Chung S, Kim S, Ryu SH, Hwang KY, Cho Y*. Mol Cells. 2020 Apr 30;43(4):350-359.

 

Azelaic Acid Induces Mitochondrial Biogenesis in Skeletal Muscle by Activation of Olfactory Receptor 544.

Thach TT, Wu C, Hwang KY, Lee SJ*. Front Physiol. 2020 Apr 17;11:329.

 

Glucose-dependent control of leucine metabolism by leucyl-tRNA synthetase 1.

Yoon I, Nam M, Kim HK, Moon HS, Kim S, Jang J, Song JA, Jeong SJ, Kim SB, Cho S, Kim Y, Lee J, Yang WS, Yoo HC, Kim K, Kim MS, Yang A, Cho K, Park HS, Hwang GS, Hwang KY, Han JM, Kim JH*, Kim S*. Science. 2020 Jan 10;367(6474):205-210.

 

Structural and kinetic insights into flavin-containing monooxygenase and calponin-homology domains in human MICAL3.

Kim J, Lee H, Roh YJ, Kim HU, Shin D, Kim S, Son J, Lee A, Kim M, Park J, Hwang SY, Kim K, Lee YK, Jung HS, Hwang KY*, Lee BC*. IUCrJ. 2020 Jan 1;7(Pt 1):90-99.

 

Development of a neutralization assay based on the pseudotyped chikungunya virus of a Korean isolate.

Chung WC, Hwang KY, Kang SJ, Kim JO, Song MJ*. J Microbiol. 2020 Jan;58(1):46-53

 

2019

Spectral and structural analysis of a red fluorescent protein from Acropora digitifera.

Kim SE, Hwang KY*, Nam KH*. Protein Sci. 2019 Feb;28(2):375-381.

 

2018

Allosteric inhibition site of transglutaminase 2 is unveiled in the N terminus.

Kim N, Kang JH, Lee WK, Kim SG, Lee JS, Lee SH, Park JB, Kim KH, Gong YD, Hwang KY*. Amino Acids. 2018 Nov;50(11):1583-1594.

 

Spectral and structural analysis of large Stokes shift fluorescent protein dKeima570.

Xu Y, Hwang KY, Nam KH*. J Microbiol. 2018 Nov;56(11):822-827.

 

Structural Analysis of an Epitope Candidate of Triosephosphate Isomerase in Opisthorchis viverrini.

Son J, Kim S, Kim SE, Lee H, Lee MR, Hwang KY*. Sci Rep. 2018 Oct 10;8(1):15075.

 

Structure-based mechanism of action of a viral poly(ADP-ribose) polymerase 1-interacting protein facilitating virus replication.

Chung WC, Kim J, Kim BC, Kang HR, Son J, Ki H, Hwang KY*, Song MJ*. IUCrJ. 2018 Oct 31;5(Pt 6):866-879

 

Structural analysis of substrate recognition by glucose isomerase in Mn²⁺ binding mode at M2 site in S. rubiginosus. Bae JE, Hwang KY, Nam KH*. Biochem Biophys Res Commun. 2018 Sep 5;503(2):770-775.

 

Dual role of SND1 facilitates efficient communication between abiotic stress signalling and normal growth in Arabidopsis.

Jeong CY, Lee WJ, Truong HA, Trịnh CS, Jin JY, Kim S, Hwang KY, Kang CS, Moon JK, Hong SW, Lee H.Jeong*. Sci Rep. 2018 Jul 4;8(1):10114.

 

Structural Basis for Inhibitor-Induced Hydrogen Peroxide Production by Kynurenine 3-Monooxygenase.

Kim HT, Na BK, Chung J, Kim S, Kwon SK, Cha H, Son J, Cho JM, Hwang KY*. Cell Chem Biol. 2018 Apr 19;25(4):426-438.

 

2017

Structural insights into the dimer-tetramer transition of FabI from Bacillus anthracis.

Kim HT, Kim S, Na BK, Chung J, Hwang E, Hwang KY*, Biochem Biophys Res Commun. 2017 Nov 4;493(1):28-33.

 

Control of leucine-dependent mTORC1 pathway through chemical intervention of leucyl-tRNA synthetase and RagD interaction.

Kim JH, Lee C, Lee M, Wang H, Kim K, Park SJ, Yoon I, Jang J, Zhao H, Kim HK, Kwon NH, Jeong SJ, Yoo HC, Kim JH, Yang JS, Lee MY, Lee CW, Yun J, Oh SJ, Kang JS, Martinis SA, Hwang KY, Guo M, Han G, Han JM*, Kim S*. Nat Commun. 2017 Sep 29;8(1):732.

 

Crystal structure of tRNA^His guanylyltransferase from Saccharomyces cerevisiae.

Lee K, Lee EH, Son J, Hwang KY*. Biochem Biophys Res Commun. 2017 Aug 19;490(2):400-405.

 

Structural insights into a 20.8-kDa tegumental-allergen-like (TAL) protein from Clonorchis sinensis.

Jo CH, Son J, Kim S, Oda T, Kim J, Lee MR, Sato M, Kim HT, Unzai S, Park SY, Hwang KY*. Sci Rep. 2017 May 11;7(1):1764.

 

The 1:2 complex between RavZ and LC3 reveals a mechanism for deconjugation of LC3 on the phagophore membrane.

Kwon DH, Kim S, Jung YO, Roh KH, Kim L, Kim BW, Hong SB, Lee IY, Song JH, Lee WC, Choi EJ, Hwang KY*, Song HK*. Autophagy. 2017 Jan 2;13(1):70-81.

 

2016

The structure of the pleiotropic transcription regulator CodY provides insight into its GTP-sensing mechanism.

Han AR, Kang HR, Son J, Kwon DH, Kim S, Lee WC, Song HK, Song MJ, Hwang KY*. Nucleic Acids Res. 2016 Nov 2;44(19):9483-9493.

 

Characterization of the sensor domain of QseE histidine kinase from Escherichia coli.

Yeo KJ, Park JW, Kim EH, Jeon YH, Hwang KY*, Cheong HK*. Protein Expr Purif. 2016 Oct;126:122-126.

 

Processing of A-form ssDNA by cryptic RNase H fold exonuclease PF2046.

Kim J, Sambalkhundev GO, Kim S, Son J, Han AR, Ko SM, Hwang KY*, Lee WC*. Arch Biochem Biophys. 2016 Sep 15;606:143-50.

 

Crystal structures of Dronpa complexed with quenchable metal ions provide insight into metal biosensor development.

Kim IJ, Kim S, Park J, Eom I, Kim S, Kim JH, Ha SC, Kim YG, Hwang KY*, Nam KH*. FEBS Lett. 2016 Sep;590(17):2982-90.

 

Essential Role of the Linker Region in the Higher Catalytic Efficiency of a Bifunctional MsrA-MsrB Fusion Protein.

Han AR, Kim MJ, Kwak GH, Son J, Hwang KY*, Kim HY*. Biochemistry. 2016 Sep 13;55(36):5117-27.

 

Structural insight into the inhibition of carbonic anhydrase by the COX-2-selective inhibitor polmacoxib (CG100649).

Kim HT, Cha H, Hwang KY*. Biochem Biophys Res Commun. 2016 Sep 9;478(1):1-6.

 

Crystal structure of Thermoplasma acidophilum XerA recombinase shows large C-shape clamp conformation and cis-cleavage mode for nucleophilic tyrosine.

Jo CH, Kim J, Han AR, Park SY, Hwang KY*, Nam KH*. FEBS Lett. 2016 Mar;590(6):848-56.

 

Analysis of Chain Branch of Polyolefins by a New Proton NMR Approach.

Jung M, Lee Y, Kwak S, Park H, Kim B, Kim S, Lee KH, Cho HS, Hwang KY*. Anal Chem. 2016 Feb 2;88(3):1516-20.

 

2015

Crystal structure of Legionella pneumophila type IV secretion system effector LegAS4.

Son J, Jo CH, Murugan RN, Bang JK, Hwang KY*, Lee WC*. Biochem Biophys Res Commun. 2015 Oct 2;465(4):817-24.

 

Kaempferol ameliorates symptoms of metabolic syndrome by regulating activities of liver X receptor-β.

Hoang MH, Jia Y, Mok B, Jun HJ, Hwang KY, Lee SJ*. J Nutr Biochem. 2015 Aug;26(8):868-75.

 

Evidence for the dimerization-mediated catalysis of methionine sulfoxide reductase A from Clostridium oremlandii.

Lee EH, Lee K, Kwak GH, Park YS, Lee KJ, Hwang KY*, Kim HY*. PLoS One. 2015 Jun 24;10(6):e0131523.

 

Selenium utilization in thioredoxin and catalytic advantage provided by selenocysteine.

Kim MJ, Lee BC, Hwang KY, Gladyshev VN, Kim HY*. Biochem Biophys Res Commun. 2015 Jun 12;461(4):648-52.

 

Mechanism of 1-Cys type methionine sulfoxide reductase A regeneration by glutaredoxin.

Kim MJ, Jeong J, Jeong J, Hwang KY, Lee KJ, Kim HY. Biochem Biophys Res Commun. 2015 Feb 20;457(4):567-71.

 

Essential role of the C-terminal helical domain in active site formation of selenoprotein MsrA from Clostridium oremlandii.

Lee EH, Lee K, Hwang KY*, Kim HY*. PLoS One. 2015 Feb 18;10(2):e0117836.

 

The dipeptide H-Trp-Arg-OH (WR) Is a PPARα agonist and reduces hepatic lipid accumulation in lipid-loaded H4IIE cells.

Jia Y, Kim JH, Nam B, Kim J, Lee JH, Kim KO, Hwang KY*, Lee SJ*. Appl Biochem Biotechnol. 2015 Jan;175(2):1211-20.

 

2014

The GSH- and GSSG-bound structures of glutaredoxin from Clostridium oremlandii.

Lee EH, Kim HY, Hwang KY*. Arch Biochem Biophys. 2014 Dec 15;564:20-5.

 

Structure of mouse muskelin discoidin domain and biochemical characterization of its self-association.

Kim KH, Hong SK, Hwang KY*, Kim EE*. Acta Crystallogr D Biol Crystallogr. 2014 Nov;70(Pt 11):2863-74. doi: 10.

 

The dual binding site of angiogenin and its inhibition mechanism: the crystal structure of the rat angiogenin-heparin complex.

Yeo KJ, Hwang E, Min KM, Jee JG, Lee CK, Hwang KY, Jeon YH, Chang SI, Cheong HK*. Chem Commun (Camb). 2014 Nov 4;50(85):12966-9.

 

Structure of the ArgRS-GlnRS-AIMP1 complex and its implications for mammalian translation.

Fu Y, Kim Y, Jin KS, Kim HS, Kim JH, Wang D, Park M, Jo CH, Kwon NH, Kim D, Kim MH, Jeon YH, Hwang KY*, Kim S*, Cho Y*. Proc Natl Acad Sci U S A. 2014 Oct 21;111(42):15084-9.

 

Structural and biochemical analysis of a type II free methionine-R-sulfoxide reductase from Thermoplasma acidophilum.

Kim HS, Kwak GH, Lee K, Jo CH, Hwang KY*, Kim HY*. Arch Biochem Biophys. 2014 Oct 15;560:10-9.

 

Structural basis of the heterodimerization of the MST and RASSF SARAH domains in the Hippo signalling pathway.

Hwang E, Cheong HK, Ul Mushtaq A, Kim HY, Yeo KJ, Kim E, Lee WC, Hwang KY, Cheong C*, Jeon YH*. Acta Crystallogr D Biol Crystallogr. 2014 Jul;70(Pt 7):1944-53.

 

The dipeptide H-Trp-Glu-OH (WE) shows agonistic activity to peroxisome proliferator-activated protein-α and reduces hepatic lipid accumulation in lipid-loaded H4IIE cells.

Jia Y, Kim JH, Nam B, Kim J, Lee JH, Hwang KY, Lee SJ*. Bioorg Med Chem Lett. 2014 Jul 1;24(13):2957-62.

 

trans-Caryophyllene is a natural agonistic ligand for peroxisome proliferator-activated receptor-α.

Wu C, Jia Y, Lee JH, Jun HJ, Lee HS, Hwang KY, Lee SJ*. Bioorg Med Chem Lett. 2014 Jul 15;24(14):3168-74.

 

The crystal structure of arginyl-tRNA synthetase from Homo sapiens.

Kim HS, Cha SY, Jo CH, Han A, Hwang KY*. FEBS Lett. 2014 Jun 27;588(14):2328-34.

 

Linalool is a PPARα ligand that reduces plasma TG levels and rewires the hepatic transcriptome and plasma metabolome.

Jun HJ, Lee JH, Kim J, Jia Y, Kim KH, Hwang KY, Yun EJ, Do KR, Lee SJ*. J Lipid Res. 2014 Jun;55(6):1098-110.

 

Structural characterization and comparison of the large subunits of IPM isomerase and homoaconitase from Methanococcus jannaschii.

Lee EH, Lee K, Hwang KY*. Acta Crystallogr D Biol Crystallogr. 2014 Apr;70(Pt 4):922-31.

 

Structural analysis of 1-Cys type selenoprotein methionine sulfoxide reductase A.

Lee EH, Kwak GH, Kim MJ, Kim HY, Hwang KY*. Arch Biochem Biophys. 2014 Mar 1;545:1-8.

 

2013

Conformational changes in human prolyl-tRNA synthetase upon binding of the substrates proline and ATP and the inhibitor halofuginone.

Son J, Lee EH, Park M, Kim JH, Kim J, Kim S, Jeon YH, Hwang KY*. Acta Crystallogr D Biol Crystallogr. 2013 Oct;69(Pt 10):2136-45.

 

Cyanidin is an agonistic ligand for peroxisome proliferator-activated receptor-alpha reducing hepatic lipid.

Jia Y, Kim JY, Jun HJ, Kim SJ, Lee JH, Hoang MH, Kim HS, Chang HI, Hwang KY, Um SJ, Lee SJ*. Biochim Biophys Acta. 2013 Apr;1831(4):698-708.

 

Efficient siRNA delivery into tumor cells by p19-YSA fusion protein.

Choi KM, Park GL, Hwang KY, Lee JW, Ahn HJ. Mol Pharm. 2013 Feb 4;10(2):763-73.

 

2012

Molecular characterization of an α-N-acetylgalactosaminidase from Clonorchis sinensis.

Lee MR, Yoo WG, Kim YJ, Kim DW, Cho SH, Hwang KY, Ju JW, Lee WJ*. Parasitol Res. 2012 Nov;111(5):2149-56.

 

Analyses of methionine sulfoxide reductase activities towards free and peptidyl methionine sulfoxides.

Kwak GH, Hwang KY*, Kim HY*. Arch Biochem Biophys. 2012 Nov 1;527(1):1-5.

 

The natural carotenoid astaxanthin, a PPAR-α agonist and PPAR-γ antagonist, reduces hepatic lipid accumulation by rewiring the transcriptome in lipid-loaded hepatocytes.

Jia Y, Kim JY, Jun HJ, Kim SJ, Lee JH, Hoang MH, Hwang KY, Um SJ, Chang HI, Lee SJ*. Mol Nutr Food Res. 2012 Jun;56(6):878-88.

 

Taurine is a liver X receptor-α ligand and activates transcription of key genes in the reverse cholesterol transport without inducing hepatic lipogenesis.

Hoang MH, Jia Y, Jun HJ, Lee JH, Hwang KY, Choi DW, Um SJ, Lee BY, You SG, Lee SJ*. Mol Nutr Food Res. 2012 Jun;56(6):900-11.

 

Crystallization and preliminary X-ray crystallographic analysis of the methionine sulfoxide reductase A domain of MsrAB from Haemophilus influenzae.

Han AR, Kim HS, Cho GY, Ki HS, Kim HY, Hwang KY*. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 May 1;68(Pt 5):557-9

 

The identification of antigenic proteins: 14-3-3 protein and propionyl-CoA carboxylase in Clonorchis sinensis.

Lee MR, Kim YJ, Kim DW, Yoo WG, Cho SH, Hwang KY, Ju JW, Lee WJ*. Mol Biochem Parasitol. 2012 Mar-Apr;182(1-2):1-6.

 

Crystal structure of LeuD from Methanococcus jannaschii.

Lee EH, Cho YW, Hwang KY*. Biochem Biophys Res Commun. 2012 Mar 9;419(2):160-4.

 

Structural analysis and serological test of arginine periplasmic binding protein 2 from Chlamydophila pneumoniae.

Park SH, Chang JE, Hawkes HJ, Kang YH, Hwang KY*. Biochem Biophys Res Commun. 2012 Feb 17;418(3):518-24.

 

2011

Ursolic acid is a PPAR-α agonist that regulates hepatic lipid metabolism.

Jia Y, Bhuiyan MJ, Jun HJ, Lee JH, Hoang MH, Lee HJ, Kim N, Lee D, Hwang KY, Hwang BY, Choi DW, Lee SJ*. Bioorg Med Chem Lett. 2011 Oct 1;21(19):5876-80.

 

Small heat shock protein as a multifunctional scaffold: integrated tumor targeting and caspase imaging within a single cage.

Choi SH, Kwon IC, Hwang KY, Kim IS, Ahn HJ*. Biomacromolecules. 2011 Aug 8;12(8):3099-106.

 

Structural insights into the metal binding properties of hypothetical protein MJ0754 from Methanococcus jannaschii.

Lee EH, Kim HS, Kim HY, Jeon YH, Hwang KY*. Proteins. 2011 Jul;79(7):2358-63.

 

Tandem use of selenocysteine: adaptation of a selenoprotein glutaredoxin for reduction of selenoprotein methionine sulfoxide reductase.

Kim MJ, Lee BC, Jeong J, Lee KJ, Hwang KY, Gladyshev VN, Kim HY*. Mol Microbiol. 2011 Mar;79(5):1194-203.

 

Crystal structure of bifunctional 5,10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase from Thermoplasma acidophilum.

Lee WH, Sung MW, Kim JH, Kim YK, Han A, Hwang KY*. Biochem Biophys Res Commun. 2011 Mar 18;406(3):459-63.

 

Crystal structures of Enoyl-ACP reductases I (FabI) and III (FabL) from B. subtilis.

Kim KH, Ha BH, Kim SJ, Hong SK, Hwang KY*, Kim EE.* J Mol Biol. 2011 Feb 25;406(3):403-15.

 

X-ray crystal structure and binding mode analysis of human S-adenosylhomocysteine hydrolase complexed with novel mechanism-based inhibitors, haloneplanocin A analogues.

Lee KM, Choi WJ, Lee Y, Lee HJ, Zhao LX, Lee HW, Park JG, Kim HO, Hwang KY, Heo YS, Choi S, Jeong LS*. J Med Chem. 2011 Feb 24;54(4):930-8.

 

2010

Development of bimolecular fluorescence complementation using Dronpa for visualization of protein-protein interactions in cells.

Lee YR, Park JH, Hahm SH, Kang LW, Chung JH, Nam KH, Hwang KY, Kwon IC, Han YS*. Mol Imaging Biol. 2010 Oct;12(5):468-78.

 

Structural insights into mouse anti-apoptotic Bcl-xl reveal affinity for Beclin 1 and gossypol.

Priyadarshi A, Roy A, Kim KS, Kim EE, Hwang KY*. Biochem Biophys Res Commun. 2010 Apr 9;394(3):515-21.

 

Crystal structures of penicillin-binding proteins 4 and 5 from Haemophilus influenzae.

Kawai F, Clarke TB, Roper DI, Han GJ, Hwang KY, Unzai S, Obayashi E, Park SY*, Tame JR*. J Mol Biol. 2010 Feb 26;396(3):634-45.

 

Structural insights into Staphylococcus aureus enoyl-ACP reductase (FabI), in complex with NADP and triclosan.

Priyadarshi A, Kim EE*, Hwang KY*. Proteins. 2010 Feb 1;78(2):480-6.

 

Structural characterization of the bifunctional glucanase-xylanase CelM2 reveals the metal effect and substrate-binding moiety.

Nam KH, Lee WH, Rhee KH, Hwang KY*. Biochem Biophys Res Commun. 2010 Jan 22;391(4):1726-30.

 

Structural insights into the substrate recognition properties of beta-glucosidase.

Nam KH, Sung MW, Hwang KY*. Biochem Biophys Res Commun. 2010 Jan 1;391(1):1131-5.

 

Crystal structure of bacterioferritin from Rhodobacter sphaeroides.

Nam KH, Xu Y, Piao S, Priyadarshi A, Lee EH, Kim HY, Jeon YH, Ha NC, Hwang KY*. Biochem Biophys Res Commun. 2010 Jan 1;391(1):990-4.

 

2009

The crystal structure of an HSL-homolog EstE5 complex with PMSF reveals a unique configuration that inhibits the nucleophile Ser144 in catalytic triads.

Nam KH, Kim SJ, Priyadarshi A, Kim HS, Hwang KY*. Biochem Biophys Res Commun. 2009 Nov 13;389(2):247-50.

 

Structural and functional analysis of Vitamin K2 synthesis protein MenD.

Priyadarshi A, Kim EE, Hwang KY*. Biochem Biophys Res Commun. 2009 Oct 30;388(4):748-51.

 

Cloning, purification and preliminary X-ray crystallographic analysis of a hypothetical protein, MJ0754, from Methanococcus jannaschii DSM 2661.

Lee EH, Nam KH, Hwang KY*. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Oct 1;65(Pt 10):1065-7.

 

Structural insights into the alanine racemase from Enterococcus faecalis.

Priyadarshi A, Lee EH, Sung MW, Nam KH, Lee WH, Kim EE*, Hwang KY*. Biochim Biophys Acta. 2009 Jul;1794(7):1030-40.

 

Identification of immunogenic antigen candidate for Chlamydophila pneumoniae diagnosis.

Park SH, Kwon SJ, Lee SJ, Kim YC, Hwang KY, Kang YH, Lee KJ*. J Proteome Res. 2009 Jun;8(6):2933-43.

 

Crystal structure of the periplasmic region of MacB, a noncanonic ABC transporter.

Xu Y, Sim SH, Nam KH, Jin XL, Kim HM, Hwang KY, Lee K, Ha NC*. Biochemistry. 2009 Jun 16;48(23):5218-25.

 

Structural and kinetic analysis of an MsrA-MsrB fusion protein from Streptococcus pneumoniae.

Kim YK, Shin YJ, Lee WH, Kim HY, Hwang KY*. Mol Microbiol. 2009 May;72(3):699-709.

 

Crystal structure of CelM2, a bifunctional glucanase-xylanase protein from a metagenome library.

Nam KH, Kim SJ, Hwang KY*. Biochem Biophys Res Commun. 2009 May 29;383(2):183-6.

 

Crystal structure of an EfPDF complex with Met-Ala-Ser based on crystallographic packing.

Nam KH, Kim KH, Kim EE, Hwang KY*. Biochem Biophys Res Commun. 2009 Apr 17;381(4):630-3.

 

Structural insights of the MenD from Escherichia coli reveal ThDP affinity.

Priyadarshi A, Saleem Y, Nam KH, Kim KS, Park SY, Kim EE, Hwang KY*. Biochem Biophys Res Commun. 2009 Mar 20;380(4):797-801.

 

Structural and functional analysis of a novel hormone-sensitive lipase from a metagenome library.

Nam KH, Kim MY, Kim SJ, Priyadarshi A, Kwon ST, Koo BS, Yoon SH, Hwang KY*. Proteins. 2009 Mar;74(4):1036-40.

 

Structural and functional analysis of a novel EstE5 belonging to the subfamily of hormone-sensitive lipase.

Nam KH, Kim MY, Kim SJ, Priyadarshi A, Lee WH, Hwang KY*. Biochem Biophys Res Commun. 2009 Feb 6;379(2):553-6.

 

Insight into the antibacterial drug design and architectural mechanism of peptide recognition from the E. faecium peptide deformylase structure.

Nam KH, Ham JI, Priyadarshi A, Kim EE, Chung N, Hwang KY*. Proteins. 2009 Jan;74(1):261-5.

 

2008

Crystallization and preliminary X-ray crystallographic analysis of the probable tRNA-modification GTPase (TrmE) from Staphylococcus aureus.

Priyadarshi A, Nam KH, Kim EE, Hwang KY*. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Dec 1;64(Pt 12):1166-8.

 

Structural studies of human brain-type creatine kinase complexed with the ADP-Mg2+-NO3- -creatine transition-state analogue complex.

Bong SM, Moon JH, Nam KH, Lee KS, Chi YM, Hwang KY*. FEBS Lett. 2008 Nov 26;582(28):3959-65.

 

Crystal structure of engineered beta-glucosidase from a soil metagenome.

Nam KH, Kim SJ, Kim MY, Kim JH, Yeo YS, Lee CM, Jun HK, Hwang KY*. Proteins. 2008 Nov 15;73(3):788-93.

 

Crystallization and preliminary X-ray diffraction analysis of 5,10- methylenetetrahydrofolate dehydrogenase/cyclohydrolase from Thermoplasma acidophilum DSM 1728.

Kim JH, Sung MW, Lee EH, Nam KH, Hwang KY*. J Microbiol Biotechnol. 2008 Feb;18(2):283-6.

 

Overexpression, crystallization, and preliminary X-ray crystallographic analysis of the alanine racemase from Enterococcus faecalis v583.

Priyadarshi A, Lee EH, Sung MW, Kim JH, Ku MJ, Kim EE, Hwang KY*. J Microbiol Biotechnol. 2008 Jan;18(1):55-8.

 

Overexpression, purification, and preliminary X-ray crystallographic studies of methionine sulfoxide reductase B from Bacillus subtilis.

Park AK, Shin YJ, Moon JH, Kim YK, Hwang KY*, Chi YM*. J Microbiol Biotechnol. 2008 Jan;18(1):59-62.

 

2007

Optimization of expression conditions for soluble protein by using a robotic system of multi-culture vessels.

Ahn WS, Ahn JY, Jung CH, Hwang KY, Kim EE, Kim J, Im H, Kim JO, Yu MH, Lee C. J Microbiol Biotechnol. 2007 Nov;17(11):1868-74.

 

Structural and mutational analysis of tRNA intron-splicing endonuclease from Thermoplasma acidophilum DSM 1728: catalytic mechanism of tRNA intron-splicing endonucleases.

Kim YK, Mizutani K, Rhee KH, Nam KH, Lee WH, Lee EH, Kim EE, Park SY, Hwang KY*. J Bacteriol. 2007 Nov;189(22):8339-46.

 

Structural basis for glutamate racemase inhibition.

Kim KH, Bong YJ, Park JK, Shin KJ, Hwang KY*, Kim EE*. J Mol Biol. 2007 Sep 14;372(2):434-43.

 

Crystal structure of the tRNA-specific adenosine deaminase from Streptococcus pyogenes.

Lee WH, Kim YK, Nam KH, Priyadarshi A, Lee EH, Kim EE, Jeon YH, Cheong C, Hwang KY*. Proteins. 2007 Sep 1;68(4):1016-9.

 

Structural characterization of the photoswitchable fluorescent protein Dronpa-C62S.

Nam KH, Kwon OY, Sugiyama K, Lee WH, Kim YK, Song HK, Kim EE, Park SY, Jeon H, Hwang KY*. Biochem Biophys Res Commun. 2007 Mar 23;354(4):962-7.

 

2006

Crystal structure of fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv.

Lee KS, Park SM, Rhee KH, Bang WG, Hwang KY*, Chi YM*. Proteins. 2006 Aug 15;64(3):817-22.

 

Expression, purification, and preliminary X-ray crystallographic analysis of the complex of G(alphai3)-RGS5 from human with GDP/Mg2+)/AlF4-.

Rhee KH, Nam KH, Lee WH, Ko YG, Kim EE, Hwang KY*. Protein Pept Lett. 2006;13(9):945-9.

 

2005

Crystallization and preliminary X-ray crystallographic studies of fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv.

Rhee KH, Lee KS, Priyadarshi A, Kim EE, Hwang KY*. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Nov 1;61(Pt 11):1017-9.

 

Crystallization and preliminary X-ray crystallographic analysis of human RGS10 complexed with Galphai3.

Lee HK, Rhee KH, Kim CW, Hwang KY*, Kim EE*. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Sep 1;61(Pt 9):831-3.

 

Crystallization and preliminary X-ray crystallographic analysis of the tRNA-specific adenosine deaminase from Streptococcus pyogenes.

Ku MJ, Lee WH, Nam KH, Rhee KH, Lee KS, Kim EE, Yu MH, Hwang KY*. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Apr 1;61(Pt 4):375-7.

 

Crystallization and preliminary X-ray crystallographic studies of glutamate racemase from Lactobacillus fermenti.

Lee KS, Park SM, Hwang KY*, Chi YM*. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Feb 1;61(Pt 2):199-201.

 

2004

Structural basis for the selective inhibition of JNK1 by the scaffolding protein JIP1 and SP600125.

Heo YS, Kim SK, Seo CI, Kim YK, Sung BJ, Lee HS, Lee JI, Park SY, Kim JH, Hwang KY, Hyun YL, Jeon YH, Ro S, Cho JM, Lee TG, Yang CH. EMBO J. 2004 Jun 2;23(11):2185-95.

 

2003

Structure of the catalytic domain of human phosphodiesterase 5 with bound drug molecules.

Sung BJ, Hwang KY, Jeon YH, Lee JI, Heo YS, Kim JH, Moon J, Yoon JM, Hyun YL, Kim E, Eum SJ, Park SY, Lee JO, Lee TG, Ro S, Cho JM. Nature. 2003 Sep 4;425(6953):98-102.

 

2002

Molecular basis for the local conformational rearrangement of human phosphoserine phosphatase.

Kim HY, Heo YS, Kim JH, Park MH, Moon J, Kim E, Kwon D, Yoon J, Shin D, Jeong EJ, Park SY, Lee TG, Jeon YH, Ro S, Cho JM, Hwang KY*. J Biol Chem. 2002 Nov 29;277(48):46651-8.

 

Interactions causing the kinetic trap in serpin protein folding.

Im H, Woo MS, Hwang KY*, Yu MH*. J Biol Chem. 2002 Nov 29;277(48):46347-54

 

Structural basis for the NAD-dependent deacetylase mechanism of Sir2.

Chang JH, Kim HC, Hwang KY, Lee JW, Jackson SP, Bell SD, Cho Y*. J Biol Chem. 2002 Sep 13;277(37):34489-98.

 

Structural basis for inhibition of protein tyrosine phosphatases by Keggin compounds phosphomolybdate and phosphotungstate.

Heo YS, Ryu JM, Park SM, Park JH, Lee HC, Hwang KY*, Kim J*. Exp Mol Med. 2002 Jul 31;34(3):211-23.

 

~2000

Crystallization and preliminary x-ray crystallographic analysis of NAD+-dependent DNA ligase from Thermus filiformis.

Lee JY, Kim HK, Chang C, Eom SH, Hwang KY, Cho Y, Yu YG, Ryu SE, Kwon ST, Suh SW. Acta Crystallogr D Biol Crystallogr. 2000 Mar;56(Pt 3):357-8.

 

Structure-based identification of a novel NTPase from Methanococcus jannaschii.
Hwang KY, Chung JH, Kim SH, Han YS, Cho Y. Nat Struct Biol. 1999 Jul;6(7):691-6.

Structure and mechanism of glutamate racemase from Aquifex pyrophilus.

Hwang KY, Cho CS, Kim SS, Sung HC, Yu YG, Cho Y. Nat Struct Biol. 1999 May;6(5):422-6.

 

Structural basis for cold adaptation. Sequence, biochemical properties, and crystal structure of malate dehydrogenase from a psychrophile Aquaspirillium arcticum

Kim SY, Hwang KY, Kim SH, Sung HC, Han YS, Cho Y. J Biol Chem. 1999 Apr 23;274(17):11761-7.

 

Expression, purification, characterization and crystallization of flap endonuclease-1 from Methanococcus jannaschii.
Bae KW, Baek KW, Cho CS, Hwang KY, Kim HR, Sung HC, Cho Y. Mol Cells. 1999 Feb 28;9(1):45-8.

 

Crystallization and preliminary x-ray analysis of glutamate racemase from Aquifex pyrophilus, a hyperthermophilic bacterium.

Hwang KY, Cho CS, Kim SS, Baek K, Kim SH, Yu YG, Cho Y. Acta Crystallogr D Biol Crystallogr. 1999 Apr;55(Pt 4):927-8.

 

The crystal structure of flap endonuclease-1 from Methanococcus jannaschii.
Hwang KY, Baek K, Kim HY, Cho Y. Nat Struct Biol. 1998 Aug;5(8):707-13.

 

Rice non-specific lipid transfer protein: the 1.6 A crystal structure in the unliganded state reveals a small hydrophobic cavity

Lee JY, Min K, Cha H, Shin DH, Hwang KY, Suh SW. J Mol Biol. 1998 Feb 20;276(2):437-48.

 

Crystal structure of carboxylesterase from Pseudomonas fluorescens, an alpha/beta hydrolase with broad substrate specificity.

Kim KK, Song HK, Shin DH, Hwang KY, Choe S, Yoo OJ, Suh SW. Structure. 1997 Dec 15;5(12):1571-84.

 

Crystal structure of thermostable alpha-amylase from Bacillus licheniformis refined at 1.7 A resolution.

Hwang KY, Song HK, Chang C, Lee J, Lee SY, Kim KK, Choe S, Sweet RM, Suh SW. Mol Cells. 1997 Apr 30;7(2):251-8.

 

The crystal structure of a triacylglycerol lipase from Pseudomonas cepacia reveals a highly open conformation in the absence of a bound inhibitor.
Kim KK, Song HK, Shin DH, Hwang KY, Suh SW. Structure. 1997 Feb 15;5(2):173-85.

 

High-resolution crystal structure of the non-specific lipid-transfer protein from maize seedlings.
Shin DH, Lee JY, Hwang KY, Kim KK, Suh SW. Structure. 1995 Feb 15;3(2):189-99.

 

Crystallization, molecular replacement solution, and refinement of tetrameric beta-amylase from sweet potato.

Cheong CG, Eom SH, Chang C, Shin DH, Song HK, Min K, Moon JH, Kim KK, Hwang KY, Suh SW. Proteins. 1995 Feb;21(2):105-17.
 

Crystallization and preliminary X-ray crystallographic study of ribosome-inactivating protein from barley seeds.

Song HK, Hwang KY, Kim KK, Suh SW. Acta Crystallogr D Biol Crystallogr. 1994 Nov 1;50(Pt 6):910-2.

 

Crystallization and preliminary X-ray crystallographic analysis of phospholipid transfer protein from maize seedlings.
Shin DH, Hwang KY, Kim KK, Kim S, Sweet RM, Suh SW.
Proteins. 1994 May;19(1):80-3.

 

Crystallization and preliminary X-ray crystallographic analysis of chitinase from barley seeds.

Song HK, Hwang KY, Kim KK, Suh SW. Proteins. 1993 Sep;17(1):107-9.

 

Crystallization and preliminary X-ray crystallographic analysis of carboxylesterase from pseudomonas fluorescens.

Kim KK, Hwang KY, Jang WH, Lee H, Yoo OJ, Choi MU, Suh SW. Arch Biochem Biophys. 1993 May;302(2):417-9.

 

Crystallization and preliminary X-ray crystallographic analysis of the protease inhibitor ecotin

Shin DH, Hwang KY, Kim KK, Lee HR, Lee CS, Chung CH, Suh SW. J Mol Biol. 1993 Feb 20;229(4):1157-8.

 

Crystallization and preliminary X-ray crystallographic analysis of arylesterase from Pseudomonas fluorescens.

Kim KK, Hwang KY, Choi KD, Kang JH, Yoo OJ, Suh SW. Proteins. 1993 Feb;15(2):213-5.

 

Crystallization and preliminary X-ray crystallographic analysis of probable amylase/protease inhibitor-B from rice seeds.

Hwang KY, Kim KK, Min K, Eom SH, Yu YG, Kim S, Sweet RM, Suh SW. J Mol Biol. 1993 Jan 5;229(1):255-7.

 

Crystallization and preliminary X-ray crystallographic analysis of alpha-amylase from Bacillus subtilis.

Chang C, Kim KK, Hwang KY, Choi MU, Suh SW. J Mol Biol. 1993 Jan 5;229(1):235-8.

 

Crystallization and preliminary X-ray crystallographic analysis of lipase from Pseudomonas cepacia.

Kim KK, Hwang KY, Jeon HS, Kim S, Sweet RM, Yang CH, Suh SW. J Mol Biol. 1992 Oct 20;227(4):1258-62.

 

Lab Profile

Proteomics, the study of protein characteristics and functions, is one of the most exciting and dynamic areas of biological research today, because it offers accessible approaches to a wide spectrum of research fields. Three dimensional structures are key element in understanding biological processes and play a central role in the discovery of new lead drugs. Due to the discovery of functional genomics, many potential drug targets are identified at tremendously fast speed. Our laboratory is focusing on the understanding the molecular mechanism of potential drug targets and nucleotide binding proteins based on the structural information. In addition we would like to extend our research to annotate the unknown proteins using functional proteomics. We would like to identify new potential drug targets using integrative-proteomics technologies.

Research Projects

• Disease-related Proteins
• Aminoacyl-tRNA Synthetase Family
• Methionin-Sulfoxide-Reductases Family
• DNA/RNA binding Proteins
• Transcription Factors
• Recombinase Family
• Virus-Host interaction Proteins
• Herpesviridae, SFTS
• Malaria Proteins

Research Scheme

준비 중입니다.